Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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Brac University
2024
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10361-229052024-05-23T21:03:52Z Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples Neha, Afra Anan Oaishi, Shahrin Sattar Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Klebsiella pneumonia Carbapenem resistance Antibiotic resistance Gene profiling Drug resistance in microorganisms Gene rearrangement This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 58-60). Klebsiella pneumoniae is a bacterium that has a rod-like structure and is classified as Gram-negative. It is a member of the Enterobacteriaceae family. This microorganism is recognized for its clinical importance, since it is frequently found in the human microbiome and has the capacity to cause respiratory, urinary, and bloodstream infections. Klebsiella pneumoniae is distinguished by its polysaccharide capsule, which enhances its ability to cause disease and common source of opportunistic infections that demonstrate resistance to antimicrobial treatments in hospitalized individuals. Carbapenem Resistance Gene Profiling in Klebsiella pneumoniae is conducted with the goal of comprehending the genetic foundation of resistance to carbapenem drugs and this focuses on the identification of distinct resistance genes found in strains of Klebsiella pneumoniae, thereby addressing the urgent problem of antibiotic resistance. In this study, a total of 27 carbapenem resistant Klebsiella pneumoniae isolates were incorporated, with 25 isolates derived from non-clinical sources, comprising vegetables and water, and 2 isolates obtained from clinical sources. The major purpose of this study was to isolate Klebsiella pneumoniae from clinical and non-clinical sources, and to analyze their antibiotic resistance profiling as well as carbapenem resistant gene profiling. The aim of this targeted approach was to identify five distinct genes linked to carbapenem resistance, specifically NDM-1, blaKPC, blaOXA-48, blaVIM, and blaIMP. Additionally, PCR was performed and upon conducting gel electrophoresis and analyzing specific data, it was observed that the detection rate for the NDM-1 gene was notably higher, with approximately 11.11% showing positive results. Among the 25 carbapenem-resistant Klebsiella pneumoniae isolates, PCR analysis revealed 2 (8%) NDM-1 positive cases from non-clinical sources and 1(50%) producers were detected from clinical sources. During the analysis, no positive result was obtained for rest of those four genes, and no bands were found within their respective ranges. Afra Anan Neha Shahrin Sattar Oaishi B. Biotechnology 2024-05-23T10:45:24Z 2024-05-23T10:45:24Z ©2023 2023-12 Thesis ID 19336017 ID 19336020 http://hdl.handle.net/10361/22905 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 30 pages application/pdf Brac University |
| institution |
Brac University |
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Institutional Repository |
| language |
English |
| topic |
Klebsiella pneumonia Carbapenem resistance Antibiotic resistance Gene profiling Drug resistance in microorganisms Gene rearrangement |
| spellingShingle |
Klebsiella pneumonia Carbapenem resistance Antibiotic resistance Gene profiling Drug resistance in microorganisms Gene rearrangement Neha, Afra Anan Oaishi, Shahrin Sattar Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
| author2 |
Haque, Fahim Kabir Monjurul |
| author_facet |
Haque, Fahim Kabir Monjurul Neha, Afra Anan Oaishi, Shahrin Sattar |
| format |
Thesis |
| author |
Neha, Afra Anan Oaishi, Shahrin Sattar |
| author_sort |
Neha, Afra Anan |
| title |
Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| title_short |
Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| title_full |
Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| title_fullStr |
Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| title_full_unstemmed |
Carbapenem resistance gene Ppofiling in Klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| title_sort |
carbapenem resistance gene ppofiling in klebsiella pneumoniae isolated from clinical and non-clinical (water and vegetables) samples |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22905 |
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