Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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Brac University
2024
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| Acceso en liña: | http://hdl.handle.net/10361/22812 |
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10361-228122024-05-14T21:03:55Z Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa Cynthia, Syeda Sadia Akther Tasnim, Homaira Hossain, M. Mahboob Department of Mathematics and Natural Sciences, Brac University Pseudomonas aeruginosa Biofilm formation Microorganism Pseudomonas aeruginosa infections--Pathogenesis Biofilms This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 49-53). The experiment started with collecting samples of Pseudomonas aeruginosa from a clinical source of burnt patients and storing them in T1N1 media at room temperature. For confirmation of identification of the supplied organism Pseudomonas aeruginosa, they were grown in different selective media, and related biochemical tests were carried out for its identification. Next, the ability of the organism to form biofilm was determined. Qualitative and quantitative methods were carried out to see if our tested organism could form biofilms. After successfully forming biofilms with the tested organism, we applied ascorbic acid solutions in different concentrations to assess the effectiveness of vitamin C in reducing the ability of these microorganisms to inhibit biofilm formation. Antibiotic susceptibility tests were also performed for the tested organism and out of six isolates used, all of them were resistant to at least seven antibiotics of three different classes. As Pseudomonas aeruginosa is resistant to antibiotics and there are concerns about its inefficacy of antibacterial medications, alternative ways can be considered to combat such microorganisms. In this study, it was observed that some concentration of ascorbic acid was able to reduce the ability of biofilm formation by Pseudomonas aeruginosa. From this study, it was found that Vitamin C at 25.6 μg/mL of concentration in the microtitre plate method and at 50 μg/ /mL concentration in the tube method could inhibit biofilm formation. Syeda Sadia Akther Cynthia Homaira Tasnim B. Microbiology 2024-05-14T04:44:55Z 2024-05-14T04:44:55Z ©2023 2023-08 Thesis ID: 18326009 ID: 18326005 http://hdl.handle.net/10361/22812 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 54 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Pseudomonas aeruginosa Biofilm formation Microorganism Pseudomonas aeruginosa infections--Pathogenesis Biofilms |
| spellingShingle |
Pseudomonas aeruginosa Biofilm formation Microorganism Pseudomonas aeruginosa infections--Pathogenesis Biofilms Cynthia, Syeda Sadia Akther Tasnim, Homaira Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Hossain, M. Mahboob |
| author_facet |
Hossain, M. Mahboob Cynthia, Syeda Sadia Akther Tasnim, Homaira |
| format |
Thesis |
| author |
Cynthia, Syeda Sadia Akther Tasnim, Homaira |
| author_sort |
Cynthia, Syeda Sadia Akther |
| title |
Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa |
| title_short |
Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa |
| title_full |
Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa |
| title_fullStr |
Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa |
| title_full_unstemmed |
Effectiveness of vitamin C on biofilm formation by clinical isolates of Pseudomonas aeruginosa |
| title_sort |
effectiveness of vitamin c on biofilm formation by clinical isolates of pseudomonas aeruginosa |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22812 |
| work_keys_str_mv |
AT cynthiasyedasadiaakther effectivenessofvitaminconbiofilmformationbyclinicalisolatesofpseudomonasaeruginosa AT tasnimhomaira effectivenessofvitaminconbiofilmformationbyclinicalisolatesofpseudomonasaeruginosa |
| _version_ |
1814309153392295936 |