Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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Brac University
2024
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10361-228112024-05-14T21:02:12Z Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka Banik, Aditya Mujtabah, Subah Basharat Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Frozen RCT foods Enterobacter spp. Klebsiella pneumoniae Antibiotic Resistance ESBL Foodborne diseases--Microbiology Pathogenic bacteria This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 45-50). This study aimed to assess the microbiological quality of frozen foods that are currently very popular in Dhaka, Bangladesh. The demand for Ready-to-Cook (RTC) frozen foods have drawn public attention dramatically all over the world, but in recent years various bacterial contamination is being detected from the frozen foods which are the cause of food spoilage and food-borne diseases. Among those various bacteria that are pathogenic, the study aimed for detecting the presence of coliform bacteria that is more specifically Enterobacter spp. and Klebsiella pneumoniae. These two types of bacteria were isolated from two different branded frozen nuggets through proper preservation and transportation methods, which was followed by proper sample collection, processing, isolation and identification. For the purpose, both the culture-based method and Most Probable Number method (MPN) method was performed and then further continued by gram staining for morphological identification, biochemical tests, Polymerase Chain Reaction (PCR), Agarose based Gel Electrophoresis, Sequencing and then lastly the Antibiotic Susceptibility testing (AST). After performing all the methodologies mentioned above, from Sample 1, 13 isolates of Enterobacter spp. and from the Sample 2, 7 isolates of Klebsiella pneumoniae were obtained. However, more in depth research is needed on the factor of isolation of Enterobacter spp. from frozen nuggets for future aspects. Aditya Banik Subah Basharat Mujtabah B. Microbiology 2024-05-14T04:25:11Z 2024-05-14T04:25:11Z ©2023 2023-10 Thesis ID: 17226011 ID: 19326009 http://hdl.handle.net/10361/22811 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 51 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Frozen RCT foods Enterobacter spp. Klebsiella pneumoniae Antibiotic Resistance ESBL Foodborne diseases--Microbiology Pathogenic bacteria |
| spellingShingle |
Frozen RCT foods Enterobacter spp. Klebsiella pneumoniae Antibiotic Resistance ESBL Foodborne diseases--Microbiology Pathogenic bacteria Banik, Aditya Mujtabah, Subah Basharat Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Haque, Fahim Kabir Monjurul |
| author_facet |
Haque, Fahim Kabir Monjurul Banik, Aditya Mujtabah, Subah Basharat |
| format |
Thesis |
| author |
Banik, Aditya Mujtabah, Subah Basharat |
| author_sort |
Banik, Aditya |
| title |
Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka |
| title_short |
Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka |
| title_full |
Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka |
| title_fullStr |
Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka |
| title_full_unstemmed |
Isolation of Enterobacter spp. and Klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in Dhaka |
| title_sort |
isolation of enterobacter spp. and klebsiella pneumoniae from ready-to-eat commercially sold frozen nuggets sold in dhaka |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22811 |
| work_keys_str_mv |
AT banikaditya isolationofenterobactersppandklebsiellapneumoniaefromreadytoeatcommerciallysoldfrozennuggetssoldindhaka AT mujtabahsubahbasharat isolationofenterobactersppandklebsiellapneumoniaefromreadytoeatcommerciallysoldfrozennuggetssoldindhaka |
| _version_ |
1814308269096697856 |