Effect of free DNA of environment surface water on bacterial biofilm formation
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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Brac University
2024
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10361-228102024-05-14T21:00:16Z Effect of free DNA of environment surface water on bacterial biofilm formation Saha, Ajora Sen, Riti Habib, Suhanin Binte Bin Naser, Iftekhar Department of Mathematics and Natural Sciences, Brac University Free DNA Surface water Bacteria Bacterial biofilms Water Biofilms Bacteria This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 58-60). Bacteriophages, commonly referred to as bacterial viruses, have long been recognized as natural adversaries of bacteria. They find application in medical settings for eliminating bacterial infections. The prevalence of bacteriophages in the environment experiences seasonal fluctuations, with an increase contributing to elevated bacterial infections and subsequent reduction in bacterial numbers, and vice versa. Bacteriophages inject their genetic material into bacterial cells, leading to replication within. Upon reaching maturity, these viral predators rupture the bacterial cell, releasing all its contents, including free DNA, into the surrounding environment. Faced with such adverse conditions, bacteria often respond by forming biofilms to protect themselves from these viral invaders. This study seeks to explore whether the free DNA introduced by Bacteriophages influences the formation of bacterial biofilms. Biofilms of a number of vibrio cholerae strains that cause diseases cholera and diarrhea during the months of May to June were subjected to free DNA. Methods of data collection and its effects were observed and analyzed using appropriate statistical analysis. The resulting data and statistical analysis suggests that there is an increase of Bacteriophage in the water which releases free DNA in the environment that causes induction of the biofilms. However, in order to provide any conclusive evidence, round the year study including more samples is required. So the presence of free DNA appears to have a discernible impact on biofilm production, highlighting its potential role in influencing bacterial behavior and community dynamics. Ajora Saha Riti Sen Suhanin Binte Habib B. Biotechnology 2024-05-14T04:06:06Z 2024-05-14T04:06:06Z ©2023 2023-11 Thesis ID 19236001 ID 19236012 ID 19236017 http://hdl.handle.net/10361/22810 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 60 pages application/pdf Brac University |
institution |
Brac University |
collection |
Institutional Repository |
language |
English |
topic |
Free DNA Surface water Bacteria Bacterial biofilms Water Biofilms Bacteria |
spellingShingle |
Free DNA Surface water Bacteria Bacterial biofilms Water Biofilms Bacteria Saha, Ajora Sen, Riti Habib, Suhanin Binte Effect of free DNA of environment surface water on bacterial biofilm formation |
description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
author2 |
Bin Naser, Iftekhar |
author_facet |
Bin Naser, Iftekhar Saha, Ajora Sen, Riti Habib, Suhanin Binte |
format |
Thesis |
author |
Saha, Ajora Sen, Riti Habib, Suhanin Binte |
author_sort |
Saha, Ajora |
title |
Effect of free DNA of environment surface water on bacterial biofilm formation |
title_short |
Effect of free DNA of environment surface water on bacterial biofilm formation |
title_full |
Effect of free DNA of environment surface water on bacterial biofilm formation |
title_fullStr |
Effect of free DNA of environment surface water on bacterial biofilm formation |
title_full_unstemmed |
Effect of free DNA of environment surface water on bacterial biofilm formation |
title_sort |
effect of free dna of environment surface water on bacterial biofilm formation |
publisher |
Brac University |
publishDate |
2024 |
url |
http://hdl.handle.net/10361/22810 |
work_keys_str_mv |
AT sahaajora effectoffreednaofenvironmentsurfacewateronbacterialbiofilmformation AT senriti effectoffreednaofenvironmentsurfacewateronbacterialbiofilmformation AT habibsuhaninbinte effectoffreednaofenvironmentsurfacewateronbacterialbiofilmformation |
_version_ |
1814306847640780800 |