Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city

This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023.

Détails bibliographiques
Auteur principal:	Haque, Nayeema
Autres auteurs:	Naser, Iftekhar Bin
Format:	Thèse
Langue:	English
Publié:	Brac University 2024
Sujets:	A. baumannii Environmental samples Biofilm ESBL Virulence factor Drug resistance
Accès en ligne:	http://hdl.handle.net/10361/22150

id	10361-22150
record_format	dspace
spelling	10361-221502024-01-15T21:02:54Z Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city Haque, Nayeema Naser, Iftekhar Bin Mahmud, Zahid Hayat Department of Mathematics and Natural Sciences, Brac University A. baumannii Environmental samples Biofilm ESBL Virulence factor Drug resistance This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023. Cataloged from PDF version of thesis. Includes bibliographical references (pages 95-113). The presence of ESBL producing Acinetobacter baumannii is a major concern for various hospital and community healthcare settings, often being linked to an increased incidence of nosocomial infections. The biofilm formation ability of A. baumannii enables it to be a persistent pathogen in these environments Furthermore, there is some evidence that environmental transport of Acinetobacter spp. plays a role in the spread of clinically relevant Acinetobacter strains in the environment. The objective of this study is to isolate ESBL positive A. baumannii from environmental samples, molecular confirmation of the presence of major ESBL and virulence genes, determination of antibiotic susceptibility profile and biofilm formation assay of the ESBL producing isolates. In this study we investigated the molecular characterization of ESBL forming A. baumannii in aquatic environment. Between April and December 2022, with 3 rounds of sample collected on 3 different seasons a total of 21 samples were collected from environmental sources and finally the study was conducted using 56 ESBL producing A. baumannii isolates. ESBL A. baumannii was isolated from those samples and the isolates were subjected to antibiotic susceptibility, PCR analysis for presence of resistance and virulence genes, and lastly biofilm formation assay. A total of 56 ESBL producing A. baumannii were isolated among them, 71.4% were positive for blaTEM gene, 5.4% were positive for blaSHV gene. Among the virulence factors, 76.8% isolates harbored the pgaB gene, 69.6% bfmS, 64.3% csuE, 62.5% ompA, 28.6% kpsMII, 26.8% ptk and epsA genes, 10.7% fimH and 5.4% bap gene. In regard to antibiotic susceptibility testing, 8.9% of the isolates were resistant to cefotaxime, 5.4% to cotrimoxazole and piperacillin/tazobactam, 1.8% tetracycline. On the other hand, 91.1% of the isolates were intermediately resistant to cefotaxime and 87.5% to ceftriaxone. Out of all the 56 isolates one isolate was found to be multi-drug resistant (MDR). In case of biofilm formation, at a temperature of 25ºC, 1.78% isolates were found to be strong biofilm formers, 7.14% isolates were moderate biofilm formers, 66.07% were weak biofilm formers. At a temperature of 37ºC, 1.78% isolates were moderate biofilm formers, 76.78% isolates weak biofilm formers. This study suggests that the ESBL producing A. baumannii is spreading from the healthcare settings to the environments at a rapid speed in Bangladesh, acting as a potential reservoir for AMR bacteria. The information may have a profound effect in contributing to the improvements in public health care policies, which are a necessity to combat the spread of antibiotic resistant strain in the environment. Nayeema Haque M. Biotechnology 2024-01-15T05:11:25Z 2024-01-15T05:11:25Z 2023 2023-08 Thesis ID 22176001 http://hdl.handle.net/10361/22150 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 124 pages application/pdf Brac University
institution	Brac University
collection	Institutional Repository
language	English
topic	A. baumannii Environmental samples Biofilm ESBL Virulence factor Drug resistance
spellingShingle	A. baumannii Environmental samples Biofilm ESBL Virulence factor Drug resistance Haque, Nayeema Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city
description	This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023.
author2	Naser, Iftekhar Bin
author_facet	Naser, Iftekhar Bin Haque, Nayeema
format	Thesis
author	Haque, Nayeema
author_sort	Haque, Nayeema
title	Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city
title_short	Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city
title_full	Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city
title_fullStr	Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city
title_full_unstemmed	Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city
title_sort	antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from goranchatbari sub-catchment in dhaka city
publisher	Brac University
publishDate	2024
url	http://hdl.handle.net/10361/22150
work_keys_str_mv	AT haquenayeema antibioticresistancevirulenceandbiofilmformingcapacityofacinetobacterbaumanniiisolatedfromgoranchatbarisubcatchmentindhakacity
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Antibiotic resistance, virulence and biofilm forming capacity of acinetobacter baumannii isolated from Goranchatbari sub-catchment in Dhaka city

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