Effectiveness of water filters used in the households of Dhaka city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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Brac University
2024
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| Acesso em linha: | http://hdl.handle.net/10361/22135 |
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10361-22135 |
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10361-221352024-02-27T09:21:46Z Effectiveness of water filters used in the households of Dhaka city Proma, Musharrat Shobnom Oieshee, Monisha Hossain Saifullah, Khaled Tasnim, Jarin Hossain, M. Mahboob Nusrat, Fariha Department of Mathematics and Natural Sciences, Brac University Water Tap Filter Fecal coliforms CFU/ml Antibiotic susceptibility Pathogenicity Biotechnology. Pathogenic bacteria Microbial contamination. Water--Purification--Filtration. Microbiology This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 54-56). This research aims to determine the microbiological quality of tap and filtered water and figure out the efficiency of filters used in the households of Dhaka city. To start, 51 individual samples of tap and filtered water from different areas were collected. The total Viable Count was done by spreading the samples on Nutrient Agar media. According to the study, the minimum TVC count for the tap water sample was 8.6×102 CFU/ml and the maximum was 3.4×105 CFU/ml, whereas the minimum TVC count for the filtered water sample was 5.7×102 CFU/ml and the maximum was 2.26×105 CFU/ml. The criterion for TVC is 1×103 CFU/ml, as stated by WHO and BDS (2009). WHO and BDS (2018) set 1×103 CFU/ml as the benchmark for TVC. Only 8 tap water samples and 15 filtered water samples were found to be within the range of WHO and BD standards based on the TVC values of all 51 samples. The Coliform count was performed after membrane filtration on MFC agar. In total, 51 tap water samples and 51 respective filtered water samples were spread over XLD and MacConkey agar plates. Among the samples, only 2 samples of tap water were not contaminated by E. coli, and only 4 samples of filtered water were not contaminated by E. coli. Next, different biochemical tests were performed to identify the organisms. After that, the research moved on to the Antibiotic Susceptibility Test with 12 different antibiotics. Afterward, a blood agar test and coagulase test were done to find out the pathogenicity of the organisms. These very tests were done to identify the pathogen more closely and precisely and to understand its species. All the suspected organisms showed Alpha hemolysis in the blood agar test, and 74% of organisms showed coagulase-positive results, which is alarming. Finally, to figure out the pathogenic organisms, PCR was performed. After the study of different types of filters, and testing the boiled water, it could be said that the boiled water showed less pathogenicity than some filters with high pathogenic rates. Musharrat Shobnom Proma Monisha Hossain Oieshee Khaled Saifullah Jarin Tasnim B. Microbiology 2024-01-14T05:11:32Z 2024-01-14T05:11:32Z 2023 2023-07 Thesis ID 18226001 ID 20336021 ID 19136028 ID 19336003 http://hdl.handle.net/10361/22135 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 60 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Water Tap Filter Fecal coliforms CFU/ml Antibiotic susceptibility Pathogenicity Biotechnology. Pathogenic bacteria Microbial contamination. Water--Purification--Filtration. Microbiology |
| spellingShingle |
Water Tap Filter Fecal coliforms CFU/ml Antibiotic susceptibility Pathogenicity Biotechnology. Pathogenic bacteria Microbial contamination. Water--Purification--Filtration. Microbiology Proma, Musharrat Shobnom Oieshee, Monisha Hossain Saifullah, Khaled Tasnim, Jarin Effectiveness of water filters used in the households of Dhaka city |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Hossain, M. Mahboob |
| author_facet |
Hossain, M. Mahboob Proma, Musharrat Shobnom Oieshee, Monisha Hossain Saifullah, Khaled Tasnim, Jarin |
| format |
Thesis |
| author |
Proma, Musharrat Shobnom Oieshee, Monisha Hossain Saifullah, Khaled Tasnim, Jarin |
| author_sort |
Proma, Musharrat Shobnom |
| title |
Effectiveness of water filters used in the households of Dhaka city |
| title_short |
Effectiveness of water filters used in the households of Dhaka city |
| title_full |
Effectiveness of water filters used in the households of Dhaka city |
| title_fullStr |
Effectiveness of water filters used in the households of Dhaka city |
| title_full_unstemmed |
Effectiveness of water filters used in the households of Dhaka city |
| title_sort |
effectiveness of water filters used in the households of dhaka city |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22135 |
| work_keys_str_mv |
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