Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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Brac University
2024
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| Διαθέσιμο Online: | http://hdl.handle.net/10361/22131 |
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10361-221312024-01-14T21:04:52Z Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north Hasan, Sakib Labonno, Afia Fahamida Haque, Mahir Ashhab Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Acintebacter baumannii Vibrio spp. Vibrio cholerae ARG ARB Hospital wastewater Water--Microbiology Cholera Vibrio infections This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 80-105). Acinetobacter baumannii is a Gram-negative bacillus which shows characteristics of obligate aerobe. It is pleomorphic and non-motile. An opportunistic and nosocomial pathogen, A. baumannii has been designated as “red alert”. This pathogen is responsible for ventilator-associated pneumonia, bloodstream infections, urinary tract infections and meningitis. As this pathogen possesses a wide range of virulence factors which make this pathogen resistant to multiple drug classes, for instance, Cephalosporins, Carbapenem, Beta-lactams etc. in recent years. On the other hand, Vibrio cholerae is Gram-negative, usually curved or comma-shaped which is the major causative agent of cholera outbreak. It has a distinct history to show resistance against a specific class of drugs (Ampicillin, Tetracycline). Wastewater from hospitals can play a significant role in dispersing pathogens to patients, community members, hospital staff, and the surrounding environment. The contagious and poisonous properties of hospital effluent make it a particularly dangerous source of antibiotic resistant gene or ARGs and antibiotic resistant bacteria or ARBs. From our study, a total of 83 samples were collected from our study sampling sites from June 2022 to January 2023, where 36 Polymerase chain reaction (PCR) confirmed Acinetobacter baumannii were obtained which was 43.37% of the sample size. In comparison to community water, isolates from hospital wastewater showed most resistance against our tested antibiotics. However, isolates from both hospital wastewater and community water showed most resistance to Cefixime (82.35%) and Azithromycin (52.94%) among 11 antibiotics. Again, a total of 75 samples were collected from the exact sampling sites from June 2022 to December 2022, where 20 PCR confirmed vibrio spp. and 17 PCR confirmed Vibrio cholerae were obtained, which accounted for 49.33% in total of the sample size. Moreover, in our study Vibrio cholerae isolates from both HWW (Hospital wastewater) and community water has shown 100% resistance towards Azithromycin and Ampicillin. The findings of our study revealed that the development of ARGs in Acinetobacter baumannii and Vibrio cholerae strains in the community setting has grown dramatically. It was expected that these ARBs and ARGs would spread from hospitals via the untreated effluents. Sakib Hasan Afia Fahamida Labonno Mahir Ashhab Haque B. Microbiology 2024-01-14T04:21:31Z 2024-01-14T04:21:31Z 2023 2023-06 Thesis ID 18226008 ID 18336003 ID 18136009 http://hdl.handle.net/10361/22131 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 105 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Acintebacter baumannii Vibrio spp. Vibrio cholerae ARG ARB Hospital wastewater Water--Microbiology Cholera Vibrio infections |
| spellingShingle |
Acintebacter baumannii Vibrio spp. Vibrio cholerae ARG ARB Hospital wastewater Water--Microbiology Cholera Vibrio infections Hasan, Sakib Labonno, Afia Fahamida Haque, Mahir Ashhab Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Ahmed, Akash |
| author_facet |
Ahmed, Akash Hasan, Sakib Labonno, Afia Fahamida Haque, Mahir Ashhab |
| format |
Thesis |
| author |
Hasan, Sakib Labonno, Afia Fahamida Haque, Mahir Ashhab |
| author_sort |
Hasan, Sakib |
| title |
Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north |
| title_short |
Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north |
| title_full |
Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north |
| title_fullStr |
Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north |
| title_full_unstemmed |
Isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in Dhaka north |
| title_sort |
isolation, characterization and comparative analysis of multi-drug resistant acinetobacter baumannii & vibrio cholerae from hospital wastewater and its adjacent community supply water in dhaka north |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22131 |
| work_keys_str_mv |
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| _version_ |
1814307016035794944 |