Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
| Κύριοι συγγραφείς: | , |
|---|---|
| Άλλοι συγγραφείς: | |
| Μορφή: | Thesis |
| Γλώσσα: | English |
| Έκδοση: |
Brac University
2024
|
| Θέματα: | |
| Διαθέσιμο Online: | http://hdl.handle.net/10361/22127 |
| id |
10361-22127 |
|---|---|
| record_format |
dspace |
| spelling |
10361-221272024-01-14T21:04:48Z Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene Adiba, Rezwana Afrin Ferdous, Riyan Mehzabin Ahmed, Akash Hasanuzzaman, Md Department of Mathematics and Natural Sciences, Brac University Pseudomonas aeruginosa Multi-drug resistance (MDR) Antibiotics,PCR Multidrug resistance This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 29-34). Introduction: Hospital wastewater is one of the most prominent sources of antibiotic-resistant pathogenic bacteria. This study aims to find multi-drug-resistant Pseudomonas aeruginosa from the hospital and nearby community water to find the organisms with Multidrug-resistant genes from hospital wastewater that get to mix with community water. Method: The study was conducted with samples from November 2022 to March 2023 and a total of 43 different isolates of Pseudomonas aeruginosa were collected from hospital wastewater and surrounding community tap water. Firstly, the phenotype technique was used to identify the multi-drug resistant isolates. Further, following the Kirby-Bauer disk diffusion method, an antibiotic susceptibility test was performed. To identify the resistant gene of ESBL (Extended spectrum β-lactamase) and MBLS (Metallo-beta-lactamase) PCR method is further performed. Result: A Total 65 Number of samples were collected from Three Hospital and their adjacent community water and Confirmed isolates of Pseudomonas aeruginosa were found in 43 of those 65 samples. The percentage of confirmed isolates is 27.95%. These 43 isolates were found resistant to Amikacin (15%), Tetracycline (46%), Amoxiclav (93%), Azithromycin (48%), Aztreonam (65%), Cefepime (32%), Cefixime (32%), Ceftazidime (100%), Chloramphenicol (11%), Ciprofloxacin (13%), Gentamicin (3%), Imipenem (11%). After that, 7 isolates were selected for further identification of Multidrug-resistant-Gene. The presence of NDM-1 was found in 3 isolates, bla TEM was found in 4 isolates, and bla VIM was found in one isolate after performing the PCR method using these 8 Genes. Conclusion: In our study 43 target Pseudomonas aeruginosa confirmed isolates were found and among them 24 were hospital isolates and 19 were community isolates. 7 MDR isolates were selected, among them 4 isolates, 3 isolates, and 1 isolate positive for respectively blaTEM, NDM- 1, and bla VIM genes. This scenario shows how hospital waste contains organisms with resistant patterns and transmitting MDR microorganisms to nearby community water and spreading MDR genes. It is becoming a serious threat to the environment and public health, so it requires proper maintenance of hospital disposal to the environment and need proper methods to prevent these strains. Rezwana Afrin Adiba Riyan Mehzabin Ferdous B. Microbiology 2024-01-14T04:12:06Z 2024-01-14T04:12:06Z 2023 2023-06 Thesis ID 18126077 ID 18126041 http://hdl.handle.net/10361/22127 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 34 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Pseudomonas aeruginosa Multi-drug resistance (MDR) Antibiotics,PCR Multidrug resistance |
| spellingShingle |
Pseudomonas aeruginosa Multi-drug resistance (MDR) Antibiotics,PCR Multidrug resistance Adiba, Rezwana Afrin Ferdous, Riyan Mehzabin Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Ahmed, Akash |
| author_facet |
Ahmed, Akash Adiba, Rezwana Afrin Ferdous, Riyan Mehzabin |
| format |
Thesis |
| author |
Adiba, Rezwana Afrin Ferdous, Riyan Mehzabin |
| author_sort |
Adiba, Rezwana Afrin |
| title |
Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| title_short |
Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| title_full |
Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| title_fullStr |
Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| title_full_unstemmed |
Characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| title_sort |
characterization of pseudomonas aeruginosa from hospital sewage water and nearby community water based on their multi-drug-resistant gene |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22127 |
| work_keys_str_mv |
AT adibarezwanaafrin characterizationofpseudomonasaeruginosafromhospitalsewagewaterandnearbycommunitywaterbasedontheirmultidrugresistantgene AT ferdousriyanmehzabin characterizationofpseudomonasaeruginosafromhospitalsewagewaterandnearbycommunitywaterbasedontheirmultidrugresistantgene |
| _version_ |
1814307509709570048 |