Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
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Brac University
2024
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10361-220712024-01-04T21:02:40Z Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil Tahmid, Md. Izhar Siddiqua, Iritra Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Keratin Minimal salt media Keratinolytic assay Soil sample Biochemical tests Bacillus licheniformis Bacillus cereus Bacillus carboniphilus Serratia marcescens Biotechnology. Chemistry. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 66-73). Keratin rich waste by-products from animal skin and other parts are being produced every year in a huge amount. These wastes are required to be degraded. Otherwise, it can become a hazardous to the environment. This study focuses to find potential bacteria from the soil sample of leather manufacturing industry located at Hazaribagh of Dhaka City, which can produce keratinase enzyme to degrade the keratin wastes. 16 individual colonies of bacteria were selected to observe the keratinolytic activity of those bacteria. Those bacteria were inoculated in minimal salt media and tested with chicken feathers onto it and results were observed with the changes in the smoothness and degradation of the feather. Only 8 bacterial colonies showed changes after keeping the solution in rotatory shaker incubator. Changes in the feather were assumed to be keratin degradation. To verify the degradation of keratin, the absorbance of the optical density of keratinolytic assay was observed of those bacteria. Different biochemical tests were done for the identification of the bacteria and the process was done with different temperature and pH separately for optimization of the experiment. Finally, extraction of DNA of those bacteria was done, along with the PCR (Polymerase Chain Reaction) to get the potential bacteria for further research. Md. Izhar Tahmid Iritra Siddiqua B. Biotechnology 2024-01-04T08:53:01Z 2024-01-04T08:53:01Z 2022 2022-08 Thesis ID: 18136080 ID: 18136005 http://hdl.handle.net/10361/22071 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 73 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Keratin Minimal salt media Keratinolytic assay Soil sample Biochemical tests Bacillus licheniformis Bacillus cereus Bacillus carboniphilus Serratia marcescens Biotechnology. Chemistry. |
| spellingShingle |
Keratin Minimal salt media Keratinolytic assay Soil sample Biochemical tests Bacillus licheniformis Bacillus cereus Bacillus carboniphilus Serratia marcescens Biotechnology. Chemistry. Tahmid, Md. Izhar Siddiqua, Iritra Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Ahmed, Akash |
| author_facet |
Ahmed, Akash Tahmid, Md. Izhar Siddiqua, Iritra |
| format |
Thesis |
| author |
Tahmid, Md. Izhar Siddiqua, Iritra |
| author_sort |
Tahmid, Md. Izhar |
| title |
Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| title_short |
Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| title_full |
Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| title_fullStr |
Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| title_full_unstemmed |
Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| title_sort |
isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22071 |
| work_keys_str_mv |
AT tahmidmdizhar isolationidentificationandcharacterizationofkeratinaseenzymeproducingbacteriacollectedfromtannerysoil AT siddiquairitra isolationidentificationandcharacterizationofkeratinaseenzymeproducingbacteriacollectedfromtannerysoil |
| _version_ |
1814306946688221184 |