Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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Brac University
2023
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10361-219602023-12-12T21:02:31Z Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city Noor, Mardia Mehzabin Godhuly, Sreemoye Sen Khan, Anika Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Biochemistry lab tests Salmonella spp. Chicken samples Resistance Sensitivity MDR MAR index PCR Microbiology This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 31-33). In Bangladesh poultry chicken is popular as a great protein source but it contains Zoonotic disease called Salmonellosis causing bacteria like Salmonella spp. Salmonellosis is a foodborne illness that is brought on by a number of non-typhoidal Salmonella enterica (NTS) serovars, primarily serovars Enteritidis and Typhimurium. The aim of this project was to Isolate the Zoonotic disease causing Salmonella and the rate of their antibiotic resistance. For this purpose 8 poultry Chicken samples were collected from different areas of Dhaka city. The highest amount of Salmonella isolates were found from flesh and that was 49.5%, then 22.7% came from cloacal swabs and 27.8% came from Liver. Antibiotic susceptibility test of these isolates was performed by using 10 antibiotics belonging to 7 groups which revealed that all the isolates (100%) were resistant to Tetracycline, Amoxicillin (93.8%), and Streptomycin (62.9%). However, commonly used antibiotics Meropenem, Imipenem and Ceftriaxone were reported to be very effective antibiotics against Salmonella spp. with sensitivity rates of 93.8%, 78.4% and 67%, respectively. This result indicates that there is a significant risk of cross-contamination and uncontrol use of different antibiotics to the poultry chicken. At the final stage PCR and Gel electrophoresis was performed for the Molecular confirmation of the Salmonella spp. Mardia Mehzabin Noor Sreemoye Sen Godhuly Anika Khan B. Microbiology 2023-12-12T04:42:07Z 2023-12-12T04:42:07Z 2023 2023-05 Thesis ID 18326020 ID 18326023 ID 18126011 http://hdl.handle.net/10361/21960 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 33 pages application/pdf Brac University |
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Brac University |
collection |
Institutional Repository |
language |
English |
topic |
Biochemistry lab tests Salmonella spp. Chicken samples Resistance Sensitivity MDR MAR index PCR Microbiology |
spellingShingle |
Biochemistry lab tests Salmonella spp. Chicken samples Resistance Sensitivity MDR MAR index PCR Microbiology Noor, Mardia Mehzabin Godhuly, Sreemoye Sen Khan, Anika Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city |
description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
author2 |
Haque, Fahim Kabir Monjurul |
author_facet |
Haque, Fahim Kabir Monjurul Noor, Mardia Mehzabin Godhuly, Sreemoye Sen Khan, Anika |
format |
Thesis |
author |
Noor, Mardia Mehzabin Godhuly, Sreemoye Sen Khan, Anika |
author_sort |
Noor, Mardia Mehzabin |
title |
Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city |
title_short |
Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city |
title_full |
Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city |
title_fullStr |
Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city |
title_full_unstemmed |
Isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of Dhaka city |
title_sort |
isolation of antibiotic resistant salmonella spp. from chicken samples collected from local wet markets of dhaka city |
publisher |
Brac University |
publishDate |
2023 |
url |
http://hdl.handle.net/10361/21960 |
work_keys_str_mv |
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_version_ |
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