Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
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Brac University
2023
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10361-217192023-10-09T21:03:02Z Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh Rahmat, Raad Naser, Iftekhar Bin Munshi, Saif Ullah Department of Mathematics and Natural Sciences, Brac University Dengue Serotypes Real time RT-PCR Prevalence Dengue infection This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 51-57). Dengue infection is a self-limiting febrile illness caused by four serotypes of dengue viruses (DENVs). It is one the most important, yet globally neglected, vector-borne diseases in tropical and subtropical regions of the world, where its etiological agent’s mosquito vectors, female Aedes aegypti and A. albopictus are habituated. Due to rapid development of the world, in terms of urbanization and international travel, dengue has been on a staggering rise with regards to geographical distribution, frequency of outbreaks, and disease severity. During the monsoon season, many of the endemic and hyperendemic countries, including Bangladesh, are vulnerable to dengue outbreaks. While it mostly leads to silent or mild to moderate symptoms, disease severity is often increased with reemergence of a serotype of DENV following years of absence, especially in cases of heterologous secondary DENV infections. This makes identification of circulating serotypes, along with detection of the prevalent serotype(s), indispensable tools during times of potential outbreaks as it may lead to nationwide preparedness, thus, enabling the control or limitation of a potential epidemic. To address the issue, this study aimed to identify the prevalence of circulating serotypes of DENV in the recent 2021 dengue outbreak in Bangladesh. In this study, suspected dengue patients were enrolled whose blood was serologically tested for the presence of dengue NS1 antigen, and anti-Dengue IgM and IgG. Following RNA extraction with RNA Isolation Kit, presence of dengue RNA was then detected in the NS1 positive samples via real-time reverse transcriptase polymerase chain reaction (RT-PCR) using a commercial Dengue Virus Real Time PCR Detection Kit. Later on, another commercial Multiplex real-time RT-PCR Dengue differentiation kit was used on the isolated RNA positive samples for serotyping the DENVs in the samples. Accordingly, this serotyping study allowed the identification of the circulating serotypes, as well as the predominant serotype in the 2021 dengue outbreak in Bangladesh. This study found that DENV2 and DENV3 were the circulating DENV serotypes in the 2021 dengue outbreak in Dhaka, Bangladesh with DENV3 being predominant. It was also revealed that majority of the cases were secondary dengue infections. Raad Rahmat B. Biotechnology 2023-10-09T04:41:39Z 2023-10-09T04:41:39Z 2022 2022-01 Thesis ID 1813608 http://hdl.handle.net/10361/21719 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 65 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Dengue Serotypes Real time RT-PCR Prevalence Dengue infection |
| spellingShingle |
Dengue Serotypes Real time RT-PCR Prevalence Dengue infection Rahmat, Raad Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Rahmat, Raad |
| format |
Thesis |
| author |
Rahmat, Raad |
| author_sort |
Rahmat, Raad |
| title |
Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh |
| title_short |
Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh |
| title_full |
Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh |
| title_fullStr |
Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh |
| title_full_unstemmed |
Detection of circulating dengue virus serotypes of 2021 dengue outbreak in Dhaka, Bangladesh |
| title_sort |
detection of circulating dengue virus serotypes of 2021 dengue outbreak in dhaka, bangladesh |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/21719 |
| work_keys_str_mv |
AT rahmatraad detectionofcirculatingdenguevirusserotypesof2021dengueoutbreakindhakabangladesh |
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1814308167145750528 |