Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses
This thesis is submitted in a partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2008
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| Լեզու: | English |
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BRAC University
2010
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| Առցանց հասանելիություն: | http://hdl.handle.net/10361/206 |
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10361-206 |
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10361-2062019-09-29T05:45:10Z Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses Khan, Md. Saifur Rahman Choudhury, Dr. Naiyyum Department of Mathematical and Natural Science, BRAC University This thesis is submitted in a partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2008 Cataloged from PDF version of thesis report. Includes bibliographical references (page 39-62). Cholera continues to be one of the most common causes of morbidity and mortality among children and adults in developing countries like Bangladesh. The aim of the study was to determine the percentage of cholera patients among the all rice watery diarrhoeal patients by their different microbiological, biochemical and serological assays. Ten percent (n=10) patients were confirmed with Vibrio choleraeOl infected. Among Vibrio cholerae isolates, 7 patients were Ogawa and 3 were Inaba. Another aim was to evaluate the natural immune responses of native cholera patients infected by Vibrio cholerae 01. LPS specific IgA response showed an increase in convalescent sera at both day 7 and day 30 compared to the baseline, acute stage on day 2. The increase in both cases were statistically significant compared to baseline (P=0.0039). When sera were tested for LPS specific IgG responses, the response in the convalescent sera at day 7 were significantly higher than at the acute sera of day 2 (P=0.002). The day 30 IgG responses were also higher and statistically significant compared to that at day 2 (P=0.0039). CTB specific IgA response at convalescent stage at day 7 was significantly increased (P=0.002). CTB specific IgA response at day 30 compared to that of the acute stage at day 2, was statistically significantly increase (P=0.0059). When sera were tested for response to CTB specific IgG, the responses of both the convalescent sera at day 7 and day 30 were found an increase compared to the response at day 2. The responses were also statistically significant (P=0.002). The vibriocidal antibody response was significantly increased at the convalescent stage on day 7 (P=0.002), and at day 30 (P=0.002). Md. Saifur Rahman Khan 2010-09-23T04:38:47Z 2010-09-23T04:38:47Z 2008 2008-09 Thesis ID 07176004 http://hdl.handle.net/10361/206 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 68 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| description |
This thesis is submitted in a partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2008 |
| author2 |
Choudhury, Dr. Naiyyum |
| author_facet |
Choudhury, Dr. Naiyyum Khan, Md. Saifur Rahman |
| format |
Thesis |
| author |
Khan, Md. Saifur Rahman |
| spellingShingle |
Khan, Md. Saifur Rahman Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| author_sort |
Khan, Md. Saifur Rahman |
| title |
Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| title_short |
Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| title_full |
Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| title_fullStr |
Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| title_full_unstemmed |
Isolation and charaterization of Vibrio cholerae O1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| title_sort |
isolation and charaterization of vibrio cholerae o1 from stools of diarrheal patients and studies of the methods to determine the immunological responses |
| publisher |
BRAC University |
| publishDate |
2010 |
| url |
http://hdl.handle.net/10361/206 |
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AT khanmdsaifurrahman isolationandcharaterizationofvibriocholeraeo1fromstoolsofdiarrhealpatientsandstudiesofthemethodstodeterminetheimmunologicalresponses |
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