Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum

This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022.

Bibliographic Details
Main Author:	Haque, S.A.M. Salman
Other Authors:	Hossain, Dr. M. Mahboob
Format:	Thesis
Language:	English
Published:	Brac University 2023
Subjects:	Endophytic fungi Pomegranate Antioxidity Fungal strains Fungal extracts Pomegranate. Medicinal plants.
Online Access:	http://hdl.handle.net/10361/19368

id	10361-19368
record_format	dspace
spelling	10361-193682023-08-09T21:02:01Z Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum Haque, S.A.M. Salman Hossain, Dr. M. Mahboob Department of Mathematics and Natural Sciences, Brac University Endophytic fungi Pomegranate Antioxidity Fungal strains Fungal extracts Pomegranate. Medicinal plants. This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022. Cataloged from PDF version of thesis. Includes bibliographical references (pages 109-114). Endophytic fungi are a group of fungi that reside in the living plant without causing any symptoms. It shows symbiotic/mutualistic characteristics with host plants. Endophytic fungi may increase the host defense system against various pathogenic microorganism and contains secondary metabolites of the respective host plant. Isolation of endophytic fungi and it’s metabolites from Punica Granatum plant grown in Bangladesh is a recent and new approach. A total of six endophytic fungi were isolated and purified from the leaf and bark part of the plant. All of the isolated endophytic fungi namely DgB1, DgB3, DgL3, and DgL4 were identified up to genus level based on macroscopic and microscopic characteristics and up to species level based on molecular characteristics as Diaporthe pseudomangiferae, Diaporthe pseudomangiferae, Phomopsis tersa, and Colletotrichum siamense respectively. Preliminary chemical screening of the fungal extracts by thin layer chromatographic technique revealed the presence of various compounds such as flavonoids, anthraquinones, couramins, isocoumarins etc. An antimicrobial study was carried out against a wide range of both Gram-positive and Gram-negative bacteria by disc diffusion method, and testing of the antioxidant property was done by following DPPH scavenging method. Fungal strain DgL4 showed significant antioxidant activity of 21.08 µg/ml and DgB1 showed moderate antimicrobial activity. The extract DgL3 was later purified by solvent treatment with mixtures of n-Hexane, DCM, and methanol. Structural elucidation of the isolated compound was done through their spectral data (NMR). Overall, these findings indicate that the medicinal plants of Bangladesh and their associated endophytic fungi could be an ideal target for the discovery of potentially bioactive compounds or lead structures for new drugs. S.A.M. Salman Haque M. Biotechnology 2023-08-09T05:56:17Z 2023-08-09T05:56:17Z 2022 2022-12 Thesis ID: 19176006 http://hdl.handle.net/10361/19368 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 114 pages application/pdf Brac University
institution	Brac University
collection	Institutional Repository
language	English
topic	Endophytic fungi Pomegranate Antioxidity Fungal strains Fungal extracts Pomegranate. Medicinal plants.
spellingShingle	Endophytic fungi Pomegranate Antioxidity Fungal strains Fungal extracts Pomegranate. Medicinal plants. Haque, S.A.M. Salman Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum
description	This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022.
author2	Hossain, Dr. M. Mahboob
author_facet	Hossain, Dr. M. Mahboob Haque, S.A.M. Salman
format	Thesis
author	Haque, S.A.M. Salman
author_sort	Haque, S.A.M. Salman
title	Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum
title_short	Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum
title_full	Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum
title_fullStr	Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum
title_full_unstemmed	Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum
title_sort	exploration of endophytic fungi and their bioactive potential associated with punica granatum
publisher	Brac University
publishDate	2023
url	http://hdl.handle.net/10361/19368
work_keys_str_mv	AT haquesamsalman explorationofendophyticfungiandtheirbioactivepotentialassociatedwithpunicagranatum
_version_	1814309651018153984

Exploration of endophytic fungi and their bioactive potential associated with Punica Granatum

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