Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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| التنسيق: | أطروحة |
| اللغة: | English |
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Brac University
2023
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/19120 |
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10361-19120 |
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10361-191202023-07-30T21:02:11Z Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city Siddique, Sifat Sarwa Noor, Shah Hasanuzzaman, Md. Department of Mathematics and Natural Sciences, Brac University Klebsiella pneumoniae Antibiotics ESBL Carbapenem Resistant Hospital Wastewater Community Klebsiella Microbiology Emerging infectious diseases This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 85-93). Klebsiella pneumoniae is one of the most occurring opportunistic microbial species responsible for pneumonia infections as well as nosocomial infections (Cordova-Espinoza et al., 2023). This species of bacteria is mostly found in the environment including soil, surface water, and medical devices (Valenzuela-Valderrama et al., 2019). Among Klebsiella pneumoniae, those strains that produce extended-spectrum beta-lactamases are important pathogens responsible for nosocomial infections which can extend to serious bacteremia and pneumonia (Filis et al., 2021). A global study has shown that around 20% to 80% of Klebsiella pneumoniae were resistant to first-line antibiotics like cephalosporins, fluoroquinolones, and aminoglycosides back in the 2000s (Pitout et al., 2015). The multi-drug resistance state of K. pneumoniae has led to the emergence of incurable diseases. In our study, we aimed to find ESBL encoding and Carbapenem-resistant Klebsiella pneumoniae isolates from the Hospital wastewater and their adjacent community tap water of Dhaka Metropolitan City. From our study of 82 confirmed K. pneumoniae isolates, 24 isolates were selected based on the phenotypic characteristics of the Antimicrobial Susceptibility Test (AST). Among these 24 isolates, 23.3% were positive for blaCTX-M and 6.7% were positive for blaTEM, blaKPC, and blaNDM-1 genes respectively. Moreover, for the SHV gene, 56.7% of isolates were positive. This ratio indicates that the bacteria K. pneumoniae acquired from both the hospital effluent and community tap water retains the ability to resist multiple classes of antibiotics. Sifat Sarwa Siddique Shah Noor B. Microbiology 2023-07-30T05:08:29Z 2023-07-30T05:08:29Z 2023 2023-06 Thesis ID 19126048 ID 19126015 http://hdl.handle.net/10361/19120 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 93 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Klebsiella pneumoniae Antibiotics ESBL Carbapenem Resistant Hospital Wastewater Community Klebsiella Microbiology Emerging infectious diseases |
| spellingShingle |
Klebsiella pneumoniae Antibiotics ESBL Carbapenem Resistant Hospital Wastewater Community Klebsiella Microbiology Emerging infectious diseases Siddique, Sifat Sarwa Noor, Shah Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Hasanuzzaman, Md. |
| author_facet |
Hasanuzzaman, Md. Siddique, Sifat Sarwa Noor, Shah |
| format |
Thesis |
| author |
Siddique, Sifat Sarwa Noor, Shah |
| author_sort |
Siddique, Sifat Sarwa |
| title |
Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_short |
Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_full |
Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_fullStr |
Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_full_unstemmed |
Detection Extended Spectrum β-Lactamase (ESBL) and Carbapenemase encoding Klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_sort |
detection extended spectrum β-lactamase (esbl) and carbapenemase encoding klebsiella pneumoniae isolates from hospital effluent wastewater and hospital adjacent community tap water in dhaka metropolitan city |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/19120 |
| work_keys_str_mv |
AT siddiquesifatsarwa detectionextendedspectrumblactamaseesblandcarbapenemaseencodingklebsiellapneumoniaeisolatesfromhospitaleffluentwastewaterandhospitaladjacentcommunitytapwaterindhakametropolitancity AT noorshah detectionextendedspectrumblactamaseesblandcarbapenemaseencodingklebsiellapneumoniaeisolatesfromhospitaleffluentwastewaterandhospitaladjacentcommunitytapwaterindhakametropolitancity |
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1814307208704294912 |