In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
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Brac University
2023
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| Online pristup: | http://hdl.handle.net/10361/18784 |
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10361-187842023-07-13T21:03:09Z In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins Fabiha, Fahmida Shadman, S. M. Salim Turjya, Rafeed Rahman Department of Mathematics and Natural Sciences, Brac University Lyssavirus Phylogroup II Glycoproteins Bats as carriers of disease. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 123-133). The genus of lyssavirus, coming from the family of Rhabdoviridae, has been around since the time of 2300 BC. A ~12kb, negative-sense RNA virus, it is known to be one of the lethal viruses ever encountered by mankind. With the advancement in the fields of genetics and bioinformatics, we have been able to classify the genus into 3 phylogroups, phylogroups I, II, & III. Available and newly engineered vaccines target the phylogroup I and III, but no significant vaccine is available for combating the phylogroup II viruses. In this study, we used immunoinformatics based approach to design a multi-epitope-based vaccine that can provide immunity against the phylogroup II lyssaviruses, Lagos Bat Virus, Mokola Bat Virus & Shimoni Bat Virus. We have identified conserved epitopes within the viral glycoprotein sequences, and constructed vaccines containing immunogenic motifs alongside these epitopes. We predicted and optimized the three-dimensional structures of our vaccines, and assessed their capacity to induce immunity. Our designed vaccines are highly antigenic, non-allergenic, and provide wide coverage. They have shown high binding affinity against MHC molecules and induced long-term immunity in immune simulation. We believe that in silico design of these vaccines is the first step in preparation against a future spread of phylogroup II lyssavirus species. Fahmida Fabiha S. M. Salim Shadman B. Biotechnology 2023-07-13T06:36:24Z 2023-07-13T06:36:24Z 2022 2022-12 Thesis ID: 18136057 ID: 17136037 http://hdl.handle.net/10361/18784 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 133 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Lyssavirus Phylogroup II Glycoproteins Bats as carriers of disease. |
| spellingShingle |
Lyssavirus Phylogroup II Glycoproteins Bats as carriers of disease. Fabiha, Fahmida Shadman, S. M. Salim In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Turjya, Rafeed Rahman |
| author_facet |
Turjya, Rafeed Rahman Fabiha, Fahmida Shadman, S. M. Salim |
| format |
Thesis |
| author |
Fabiha, Fahmida Shadman, S. M. Salim |
| author_sort |
Fabiha, Fahmida |
| title |
In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins |
| title_short |
In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins |
| title_full |
In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins |
| title_fullStr |
In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins |
| title_full_unstemmed |
In silico design of a multi-epitope vaccine against Lyssavirus Phylogroup II Glycoproteins |
| title_sort |
in silico design of a multi-epitope vaccine against lyssavirus phylogroup ii glycoproteins |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18784 |
| work_keys_str_mv |
AT fabihafahmida insilicodesignofamultiepitopevaccineagainstlyssavirusphylogroupiiglycoproteins AT shadmansmsalim insilicodesignofamultiepitopevaccineagainstlyssavirusphylogroupiiglycoproteins |
| _version_ |
1814309215559221248 |