Effects Of horizontal gene transfer on bacteriophage infectivity
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Brac University
2023
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10361/18780 |
| id |
10361-18780 |
|---|---|
| record_format |
dspace |
| spelling |
10361-187802023-07-13T21:02:44Z Effects Of horizontal gene transfer on bacteriophage infectivity Bushra, Adiba Effat Islam, Farzana Bin Naser, Iftekhar Department of Mathematics and Natural Sciences, Brac University Gene transfer Bacteriophage infectivity Transgenic organisms. Genetic transformation. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 61-66). Vibrio cholerae is one of the very well-known and common pathogenic bacteria which cause mild to severe gastrointestinal diseases, and in extreme cases, patients die. Specially children are victims where proper clinical,environmental, and public health support are insufficient. As a treatment, antibiotics were promising until vibrio cholerae became multidrug resistant by raising a global concern. In our environment many antibiotic resistant genes are increasing day by day. Following previous successful studies, bacteriophage therapy for antibiotic resistant vibrio strains is considered as one of the potential solutions to such problems. In this study, six native strains of vibrio cholerae from the environment underwent antibiotic resistant transformation. The main purpose of this study is to observe the infectivity of selected bacteriophages on strains of similar genetic makeup following antibiotic resistant traits, especially ampicillin and kanamycin antibiotics. In other words, to find the potentiality of phage therapy. A positive finding will be beneficial for vibrio cholera treatment and contribute to clinical, environmental biotechnology and public health as well. Therefore, by isolating antibiotic resistant genes containing DNA, transforming those into native vibrio strains and finally double layer spot test on the prepared strains gave a conclusive and intriguing outcome of phage infectivity which favors future studies with more antibiotic resistant vibrio strains and bacteriophages. Adiba Effat Bushra Farzana Islam Tasmia Rahman Ratri B. Biotechnology 2023-07-13T06:10:16Z 2023-07-13T06:10:16Z 2022 2022-12 Thesis ID: 18136001 ID: 18136003 ID: 18136053 http://hdl.handle.net/10361/18780 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 66 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Gene transfer Bacteriophage infectivity Transgenic organisms. Genetic transformation. |
| spellingShingle |
Gene transfer Bacteriophage infectivity Transgenic organisms. Genetic transformation. Bushra, Adiba Effat Islam, Farzana Effects Of horizontal gene transfer on bacteriophage infectivity |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Bin Naser, Iftekhar |
| author_facet |
Bin Naser, Iftekhar Bushra, Adiba Effat Islam, Farzana |
| format |
Thesis |
| author |
Bushra, Adiba Effat Islam, Farzana |
| author_sort |
Bushra, Adiba Effat |
| title |
Effects Of horizontal gene transfer on bacteriophage infectivity |
| title_short |
Effects Of horizontal gene transfer on bacteriophage infectivity |
| title_full |
Effects Of horizontal gene transfer on bacteriophage infectivity |
| title_fullStr |
Effects Of horizontal gene transfer on bacteriophage infectivity |
| title_full_unstemmed |
Effects Of horizontal gene transfer on bacteriophage infectivity |
| title_sort |
effects of horizontal gene transfer on bacteriophage infectivity |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18780 |
| work_keys_str_mv |
AT bushraadibaeffat effectsofhorizontalgenetransferonbacteriophageinfectivity AT islamfarzana effectsofhorizontalgenetransferonbacteriophageinfectivity |
| _version_ |
1814307141770543104 |