HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2022.
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Brac University
2023
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| 在线阅读: | http://hdl.handle.net/10361/18747 |
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10361-187472023-07-12T21:04:53Z HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample Khan, Abrar Kabir Haque, Dr. Md. Aminul Department of Pharmacy, Brac University Andrias davidianus Antimicrobial peptide Magnetic cell membrane separation RP-HPLC Anti-infective agents This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2022. Cataloged from PDF version of thesis. Includes bibliographical references (pages 26-32). The Andrias davidianus has a long history of use in traditional Chinese medicine. Its blood is regarded as a byproduct or waste of the meat industry. Although reports of the isolation of antimicrobial peptides from various sources exist, there are no records of their isolation from the blood of A. davidianus. In this study, Andricin B, an antimicrobial peptide, was extracted from A. davidianus blood using a novel technique that combines magnetic liposome adsorption with reversed-phase high-performance liquid chromatography. Further research was done on the structure, antibacterial capability, and safety of Andricin B. N-terminal sequencing was used to determine the amino acid sequence, which was discovered to be Gly-Leu-Thr-Arg-Leu-Phe-Ser Val-Ile-Lys. A clearly defined random coil conformation was proposed by CD spectra and the three-dimensional structure prediction made by bioinformatics tools. All of the bacteria tested in this study's Andricin B tests, as well as some fungi, were shown to be resistant to it. The MICs, or minimal inhibitory concentrations, ranged from 8 to 64 g ml-1. Additionally, the haemolytic testing indicated that Andricin B might be regarded as safe at the MICs. Finally, it was discovered that Andricin B prevented Staphylococcus aureus from growing in cooked A. davidianus flesh. This work demonstrates that Andricin B is a promising new antimicrobial peptide that may offer additional insights into the creation of novel medications. Abrar Kabir Khan B. Pharmacy 2023-07-12T05:24:09Z 2023-07-12T05:24:09Z 2022 2022-10 Thesis ID: 18146008 http://hdl.handle.net/10361/18747 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 32 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Andrias davidianus Antimicrobial peptide Magnetic cell membrane separation RP-HPLC Anti-infective agents |
| spellingShingle |
Andrias davidianus Antimicrobial peptide Magnetic cell membrane separation RP-HPLC Anti-infective agents Khan, Abrar Kabir HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2022. |
| author2 |
Haque, Dr. Md. Aminul |
| author_facet |
Haque, Dr. Md. Aminul Khan, Abrar Kabir |
| format |
Thesis |
| author |
Khan, Abrar Kabir |
| author_sort |
Khan, Abrar Kabir |
| title |
HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample |
| title_short |
HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample |
| title_full |
HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample |
| title_fullStr |
HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample |
| title_full_unstemmed |
HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample |
| title_sort |
hplc in the purification of antimicrobial peptides from andrias davidianus blood sample |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18747 |
| work_keys_str_mv |
AT khanabrarkabir hplcinthepurificationofantimicrobialpeptidesfromandriasdavidianusbloodsample |
| _version_ |
1814309436171223040 |