Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
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Brac University
2023
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10361-187212023-07-11T21:05:05Z Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae Zaman, Ifthikhar Al Shahariar, Mahruf Howlader Emu, MD. Rezanur Rahman Hossain, Dr. M. Mahboob Department of Mathematics and Natural Sciences, Brac University Plastic bioremediation Soil bacteria Superworm Zophobas morio Larvae Plastic scrap--Environmental aspects. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 53-56). Plastic is a recalcitrant molecule that cannot be easily biodegraded. As a result, almost all the plastic that has been manufactured is still in the world. There are microplastics in everywhere like air, water, soil, and specialty foods. A recent discovery suggests we have microplastics in our blood and even microplastics can be transferred from mother to newborn child. There are many types of plastics, and in this research three types of plastics were used, Polyethylene (PE), Low-Density Polyethylene (LDPE), and Expanded Polystyrene (EPS). Two types of samples were used, soil and Zophobas morio larvae. From the soil sample, three types of bacteria were isolated and identified using biochemical tests and ABIS online software where Prolinoborus fasciculus was predominant. These bacteria remained alive for the last six months of incubation in minimal salt broth with only the carbon source being PE. On the other hand, Zophobas morio, a super worm, was used to find out the biodegradation capability in the larvae stage of the insect and also larvae feces bacteria that is responsible for aiding the larvae in digesting the plastics. The larvae consumed 47.07% LDPE, 30.51% EPS, and 26.32% PE in about two months, and in the duration of the experiment no larvae died and they were also seen to recycle their feces. From feces, four bacteria were isolated according to colony morphology that was incubated in minimal salt broth for two months with the sole carbon source being the aforementioned particular plastics, and by doing 16s rRNA sequencing, Pseudomonas guariconensis, and Pseudomonas aeruginosa were identified. Ifthikhar Zaman Mahruf Al Shahariar MD. Rezanur Rahman Howlader Emu B. Biotechnology 2023-07-11T05:50:02Z 2023-07-11T05:50:02Z 2022 2022-12 Thesis ID: 19136034 ID: 19136043 ID: 18336018 http://hdl.handle.net/10361/18721 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 56 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Plastic bioremediation Soil bacteria Superworm Zophobas morio Larvae Plastic scrap--Environmental aspects. |
| spellingShingle |
Plastic bioremediation Soil bacteria Superworm Zophobas morio Larvae Plastic scrap--Environmental aspects. Zaman, Ifthikhar Al Shahariar, Mahruf Howlader Emu, MD. Rezanur Rahman Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Hossain, Dr. M. Mahboob |
| author_facet |
Hossain, Dr. M. Mahboob Zaman, Ifthikhar Al Shahariar, Mahruf Howlader Emu, MD. Rezanur Rahman |
| format |
Thesis |
| author |
Zaman, Ifthikhar Al Shahariar, Mahruf Howlader Emu, MD. Rezanur Rahman |
| author_sort |
Zaman, Ifthikhar |
| title |
Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae |
| title_short |
Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae |
| title_full |
Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae |
| title_fullStr |
Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae |
| title_full_unstemmed |
Different types of plastic bioremediation using soil bacteria and Superworm zophobas morio larvae |
| title_sort |
different types of plastic bioremediation using soil bacteria and superworm zophobas morio larvae |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18721 |
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