In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
| Príomhchruthaitheoir: | |
|---|---|
| Rannpháirtithe: | |
| Formáid: | Tráchtas |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
Brac University
2023
|
| Ábhair: | |
| Rochtain ar líne: | http://hdl.handle.net/10361/18634 |
| id |
10361-18634 |
|---|---|
| record_format |
dspace |
| spelling |
10361-186342023-06-18T21:02:17Z In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease Tanim, Tahsin Mahmud Sayem, Mohammed Department of Mathematics and Natural Sciences, Brac University Autoimmune disease Borrelia burgdorferi Bioinformatics Molecular mimicry Antigen Infection MHCII binding epitopes Homologous Biotechnology This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 42-50). Autoimmune disorder is a dysfunctional immune system in a body, which attacks its own cells. Borrelia burgdorferi, a pathogenic bacterium, shares an intrinsic relation to Lyme disease in humans causing autoreactive responses that remained a serious health concern for the public. Molecular mimicry is one of the primary mechanisms by which pathogen or toxic chemicals induce autoimmunity. In this research, a pipeline of bioinformatics tools was applied for examining the molecular mimicry between human and B. burgdorferi protein sequences from a structural perspective. In addition, the potential antigenic peptides of this bacteria similar to the peptides of human were classified and the fundamental molecular mechanisms of autoimmune disorders were analyzed. To initiate the study, proteome databases were retrieved and removed their paralogous sequences. Subsequently, the homologous proteins were identified along with enrichment pathways. Then, the subcellular localization of proteins were predicted followed by the prediction of MHC class II epitopes. Additionally, the 3D structure of the epitopes was modelled for comparing the structure of host and pathogen. The bacterial epitopes DFTIEVERSLRVLDG, LRLKKLIIDIMSNQI and VIAQLLFLESEDSSK that superimposed with the respective host epitopes DFTIEVERALRVLDG, MKLKKQLYNIYAKHT and VIAQLLFLQSESNKK showed 100% binding which were highly predicted cross reactivity. Finally, docking scores of these bacterial epitopes revealed the binding affinity towards the host HLA alleles including DRB1*07:01, DRB1*01:01and DRB4*01:01 that may lead to autoimmune disorders in humans caused by B. burgdorferi infection. Tahsin Mahmud Tanim B. Biotechnology 2023-06-18T06:36:09Z 2023-06-18T06:36:09Z 2022 2022-12 Thesis ID 18236010 http://hdl.handle.net/10361/18634 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 50 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Autoimmune disease Borrelia burgdorferi Bioinformatics Molecular mimicry Antigen Infection MHCII binding epitopes Homologous Biotechnology |
| spellingShingle |
Autoimmune disease Borrelia burgdorferi Bioinformatics Molecular mimicry Antigen Infection MHCII binding epitopes Homologous Biotechnology Tanim, Tahsin Mahmud In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Sayem, Mohammed |
| author_facet |
Sayem, Mohammed Tanim, Tahsin Mahmud |
| format |
Thesis |
| author |
Tanim, Tahsin Mahmud |
| author_sort |
Tanim, Tahsin Mahmud |
| title |
In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease |
| title_short |
In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease |
| title_full |
In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease |
| title_fullStr |
In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease |
| title_full_unstemmed |
In silico identification of molecular mimicry between T-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in Lyme disease |
| title_sort |
in silico identification of molecular mimicry between t-cell epitopes of borrelia burgdorferi and the human proteome: implications for autoimmune response in lyme disease |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18634 |
| work_keys_str_mv |
AT tanimtahsinmahmud insilicoidentificationofmolecularmimicrybetweentcellepitopesofborreliaburgdorferiandthehumanproteomeimplicationsforautoimmuneresponseinlymedisease |
| _version_ |
1814308980398227456 |