Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
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Brac University
2023
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10361-182862023-05-15T21:01:51Z Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample Purkaystha, Anirban Megha, Sumiya Al Sadia Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Degradation Decolourization Biodegradability Bacillus spp. Bacillus subtilis Staphylococcus hominis This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 36-38). Textile and dyeing industry’s effluents and wastes are considered one of the top water pollutants in Bangladesh which ultimately leads to public health hazard. This study intends to explore the capacity of local bacteria to degrade dye and the roles of pH concentration and temperature in facilitating Azo dye and Reactive Dye degradation. Samples from river sludge were taken from the Dhaleshwari and Turag rivers and serially diluted. Then bacterial isolation was randomly selected based on morphology and each individual colony of focus was inoculated in dye-mixed nutritional broth. Colonies were cultured for seven days at various temperatures and had different pH concentrations to study biodegradability. To determine the actual decolourizationn (%) amount, optical density values were measured for each sample in the meanwhile. All microorganisms demonstrated successful biodegradability for selected dyes. Biochemical tests were carried out for the identification of the bacterial strains. This study found that Bacillus spp. degraded azo dye at a rate of 69.26% at pH 6 & 37 ̊c. Furthermore, Bacillus spp. Attained 52.70 % degradation at pH 6 and 45 °c. The results disclosed that Bacillus spp. were the most efficient in degrading reactive and azo dyes, followed by Bacillus subtilis and Staphylococcus hominis. This study can further instigate the scope of the biodegradability of dye to be ready for commercial usage for effluent treatment. Anirban Purkaystha Sumiya Al Sadia Megha B. Microbiology 2023-05-15T05:08:22Z 2023-05-15T05:08:22Z 2022 2022-11 Thesis ID 18126047 ID 18126071 http://hdl.handle.net/10361/18286 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 38 pages application/pdf Brac University |
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Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Degradation Decolourization Biodegradability Bacillus spp. Bacillus subtilis Staphylococcus hominis |
| spellingShingle |
Degradation Decolourization Biodegradability Bacillus spp. Bacillus subtilis Staphylococcus hominis Purkaystha, Anirban Megha, Sumiya Al Sadia Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. |
| author2 |
Ahmed, Akash |
| author_facet |
Ahmed, Akash Purkaystha, Anirban Megha, Sumiya Al Sadia |
| format |
Thesis |
| author |
Purkaystha, Anirban Megha, Sumiya Al Sadia |
| author_sort |
Purkaystha, Anirban |
| title |
Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| title_short |
Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| title_full |
Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| title_fullStr |
Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| title_full_unstemmed |
Isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| title_sort |
isolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sample |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18286 |
| work_keys_str_mv |
AT purkaysthaanirban isolationidentificationandefficacyassessmentofbacteriadegradingazodyereactivedyefromriversludgesample AT meghasumiyaalsadia isolationidentificationandefficacyassessmentofbacteriadegradingazodyereactivedyefromriversludgesample |
| _version_ |
1814309576727592960 |