The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet

This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.

Detalles Bibliográficos
Main Authors:	Alam, Afia Salsabil, Zanzabil, Khwaja Zohura, Mahmood, Maiesha Samiha
Outros autores:	Hossain, Mahboob
Formato:	Thesis
Idioma:	English
Publicado:	Brac University 2023
Subjects:	De-inking Effluent Pollution Paper industry Bacterial diseases
Acceso en liña:	http://hdl.handle.net/10361/18281

id	10361-18281
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spelling	10361-182812023-05-15T21:01:45Z The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet Alam, Afia Salsabil Zanzabil, Khwaja Zohura Mahmood, Maiesha Samiha Hossain, Mahboob Department of Mathematics and Natural Sciences, Brac University De-inking Effluent Pollution Paper industry Bacterial diseases This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 59-63). The wastage of paper and ink is a major cause of pollution in Bangladesh. The ever-rising population demands an increased requirement for paper and ink that often end up occupying landfills or contributing to air pollution. However, the current methods of paper recycling involve paper de-inking, which is largely dependent on chemical means that further aggravates this problem. The objective of this research was to find an alternative biological solution to the chemical de-inking process. Bacteria capable of decolorizing ink were identified and isolated using media that contained ink as the only carbon source. Five samples of bacteria capable of efficient decolorization were found with decolorization percentages of 42.93%, 39.75%, 37.95%, 37.55%, and 30.02% for isolates RGS-1, SPS-1, RGS-2, SPS-2, and SPS-3, respectively. Isolates SPS-1, SPS-2, and SPS-3 also exhibited cellulolytic activity. Protein crystals from the bacterial culture supernatant were precipitated and purified using ammonium sulfate precipitation followed by snake-skin dialysis respectively, after which de-inking activity increased. The optimal de-inking was observed when extracellular and intracellular extracts were combined and added to ink; isolate SPS-2 showed a decolorization percentage of 70.75%. The bacteria provide an avenue of research for the sustainable recycling of paper, thereby alleviating the climate problem. Afia Salsabil Alam Khwaja Zohura Zanzabil Maiesha Samiha Mahmood B. Biotechnology 2023-05-15T03:44:08Z 2023-05-15T03:44:08Z 2022 2022-12 Thesis ID 19136047 ID 18236026 ID 19136023 http://hdl.handle.net/10361/18281 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 63 pages application/pdf Brac University
institution	Brac University
collection	Institutional Repository
language	English
topic	De-inking Effluent Pollution Paper industry Bacterial diseases
spellingShingle	De-inking Effluent Pollution Paper industry Bacterial diseases Alam, Afia Salsabil Zanzabil, Khwaja Zohura Mahmood, Maiesha Samiha The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet
description	This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
author2	Hossain, Mahboob
author_facet	Hossain, Mahboob Alam, Afia Salsabil Zanzabil, Khwaja Zohura Mahmood, Maiesha Samiha
format	Thesis
author	Alam, Afia Salsabil Zanzabil, Khwaja Zohura Mahmood, Maiesha Samiha
author_sort	Alam, Afia Salsabil
title	The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet
title_short	The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet
title_full	The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet
title_fullStr	The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet
title_full_unstemmed	The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet
title_sort	isolation and characterization of bacteria exhibiting deinking activity from regions in sylhet
publisher	Brac University
publishDate	2023
url	http://hdl.handle.net/10361/18281
work_keys_str_mv	AT alamafiasalsabil theisolationandcharacterizationofbacteriaexhibitingdeinkingactivityfromregionsinsylhet AT zanzabilkhwajazohura theisolationandcharacterizationofbacteriaexhibitingdeinkingactivityfromregionsinsylhet AT mahmoodmaieshasamiha theisolationandcharacterizationofbacteriaexhibitingdeinkingactivityfromregionsinsylhet AT alamafiasalsabil isolationandcharacterizationofbacteriaexhibitingdeinkingactivityfromregionsinsylhet AT zanzabilkhwajazohura isolationandcharacterizationofbacteriaexhibitingdeinkingactivityfromregionsinsylhet AT mahmoodmaieshasamiha isolationandcharacterizationofbacteriaexhibitingdeinkingactivityfromregionsinsylhet
_version_	1814307277853687808

The isolation and characterization of bacteria exhibiting deinking activity from regions in Sylhet

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