Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
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| التنسيق: | أطروحة |
| اللغة: | English |
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Brac University
2023
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/18244 |
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10361-18244 |
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10361-182442023-05-08T21:01:54Z Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria Prapti, Jarin Tusnim Sumaiya, Kazi Maliha, Farhat Bin Naser, Dr. Iftekhar Department of Mathematics and Natural Sciences, Brac University Antibiotic resistance Origanum vulgare L OEO Azadirachta indica Pathogenic bacteria. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 59-65). Antibiotic resistance has been on the rise for many years now. Multidrug resistance patterns in Gramnegative and Gram-positive bacterial strains are difficult to cure and might even be untreatable with conventional synthetic antibiotics. Furthermore, there is a shortage of novel antibiotics in the market. Therefore, to combat global health crisis, development of new treatment options and alternative antimicrobial therapies, preferably from natural sources, are essential. Oregano (Origanum vulgare L) essential oil (OEO) is known to have antibacterial properties and this study was aimed to find its ability to inhibit the growth of some infectious bacterial strains and also do a comparative analysis with a well-known antibacterial oil; Neem (Azadirachta indica) essential oil. This study on antibacterial activity against selected pathogens was carried out using Kill-time and Dosage dependent spread plate method. Klebsiella pneumonia, shigella, STEC, Salmonella typhi, Aeromonas hydrophila, Staphylococcus aureus and Enterococcus faecalis was selected for this study. The study showed the average percentage inhibition of each oil is directly proportional with the dosage. OEO showed greater antibacterial activity against the bacterial strains as compared to NEO. For 50µl dosage the average inhibition rate for OEO was 73.3% and for NEO it was only 45.9%. NEO was less effective on bacterial strains; Klebsiella pneumonia, Shigella, Aeromonas hydrophia, STEC, Staphylococcus aureus and Enterococcus faecalis as compared to OEO. The only bacterial strain NEO was more effective on was Salmonella typhi. OEO demonstrated 100% inhibition against Klebsiella pneumonia, Shigella, Aeromonas hydrophila, STEC, Staphylococcus Aureus and Enterococcus faecalis at varying dosage of oil. Whereas, NEO showed 100% inhibition against Salmonella typhi and Staphylococcus Aureus at 150µl and 200µl dosage of oil respectively. Jarin Tusnim Prapti Kazi Sumaiya Farhat Maliha B. Biotechnology 2023-05-08T09:25:27Z 2023-05-08T09:25:27Z 2022 2022-10 Thesis ID: 17136016 ID: 17336006 ID: 17336009 http://hdl.handle.net/10361/18244 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 70 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Antibiotic resistance Origanum vulgare L OEO Azadirachta indica Pathogenic bacteria. |
| spellingShingle |
Antibiotic resistance Origanum vulgare L OEO Azadirachta indica Pathogenic bacteria. Prapti, Jarin Tusnim Sumaiya, Kazi Maliha, Farhat Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Bin Naser, Dr. Iftekhar |
| author_facet |
Bin Naser, Dr. Iftekhar Prapti, Jarin Tusnim Sumaiya, Kazi Maliha, Farhat |
| format |
Thesis |
| author |
Prapti, Jarin Tusnim Sumaiya, Kazi Maliha, Farhat |
| author_sort |
Prapti, Jarin Tusnim |
| title |
Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria |
| title_short |
Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria |
| title_full |
Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria |
| title_fullStr |
Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria |
| title_full_unstemmed |
Anti-bacterial effect of essential oil extracted from homegrown Origanum vulgare L and Azadirachta indica tree on pathogenic bacteria |
| title_sort |
anti-bacterial effect of essential oil extracted from homegrown origanum vulgare l and azadirachta indica tree on pathogenic bacteria |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18244 |
| work_keys_str_mv |
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| _version_ |
1814308900820746240 |