Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022.
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Brac University
2023
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10361-181482023-04-13T21:01:45Z Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms Hasan, Md Rockybul Bin Naser, Iftekhar Department of Mathematics and Natural Sciences, Brac University Vibrio cholerae Biofilms Bacteriophage Antibiotic Vibrio cholerae. This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022. Cataloged from PDF version of thesis. Includes bibliographical references (pages 40-47). Vibrio cholerae is a gram-negative coccobacillus known as the causative agent of cholera- a gastrointestinal disease endemic to developing and underdeveloped countries, Bangladesh being one of them. The ability to form biofilms made it very difficult for many broad host range antibiotics to penetrate through them and destroy them completely. The cells in the biofilm can persist in a wide range of environmental conditions by remaining metabolically dormant and able to resuscitate into planktonic cells after getting suitable opportunities which can lead to a cholera outbreak. Antibiotics have been used to breakdown biofilms, however the attempts remained unsuccessful in most cases even if the concentration is a few times higher compared to the planktonic counterpart of the same bacteria. In this study, bacteriophage therapy was introduced as an alternative to antibiotic treatment for degrading V. cholerae biofilms. Combined treatment of phage and antibiotic (kanamycin) was performed to check the effectiveness in degrading biofilms and killing the planktonic bacteria at the same time. In both cases of single phage and combination treatment there were evidence of decreasing the biofilm layers of Vibrio strains but not when treated with kanamycin alone. Shiga Toxin producing E. coli (STEC) was used to screen the phage’s ability to target hosts other than Vibrio strains, which turned out to be ineffective. There is potential scope to use combined phage and antibiotic therapy in regulating biofilm formation, but further investigation and risk evaluation is required Md Rockybul Hasan M. Biotechnology 2023-04-13T08:24:06Z 2023-04-13T08:24:06Z 2022 2022-11 Thesis ID: 20376002 http://hdl.handle.net/10361/18148 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 49 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Vibrio cholerae Biofilms Bacteriophage Antibiotic Vibrio cholerae. |
| spellingShingle |
Vibrio cholerae Biofilms Bacteriophage Antibiotic Vibrio cholerae. Hasan, Md Rockybul Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022. |
| author2 |
Bin Naser, Iftekhar |
| author_facet |
Bin Naser, Iftekhar Hasan, Md Rockybul |
| format |
Thesis |
| author |
Hasan, Md Rockybul |
| author_sort |
Hasan, Md Rockybul |
| title |
Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| title_short |
Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| title_full |
Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| title_fullStr |
Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| title_full_unstemmed |
Degrading Vibrio cholerae Biofilms with Bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| title_sort |
degrading vibrio cholerae biofilms with bacteriophage and potential combined effect of phage and antibiotic on biofilms |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18148 |
| work_keys_str_mv |
AT hasanmdrockybul degradingvibriocholeraebiofilmswithbacteriophageandpotentialcombinedeffectofphageandantibioticonbiofilms |
| _version_ |
1814308151024943104 |