Rapid detection of viable Vibrio Cholerae by Bacteriophage
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.
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| स्वरूप: | थीसिस |
| भाषा: | English |
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Brac University
2023
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| ऑनलाइन पहुंच: | http://hdl.handle.net/10361/18043 |
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10361-18043 |
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10361-180432023-04-02T21:01:44Z Rapid detection of viable Vibrio Cholerae by Bacteriophage Rashid, Nafisa Ul Haque, Naim Sakib, Ahsan Bin Naser, Iftekhar Department of Mathematics and Natural Sciences, Brac University Cell viability Detection methods Foodborne pathogens Rapid methods Viable-but-nonculturable. Vibrio infections This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 38-40). For the sake of food safety and public health, it is crucial to be able to quickly identify live viruses in food. Traditional methods of establishing microbial viability, known as “culture-based detection methods” can be difficult, time-consuming, and slowly produce outcomes. In recent years, several culture-free techniques to identify live viruses have been published, including both based on nucleic acids (PCR combined with the use of cell viability dyes or reverse-transcriptase PCR to detect messenger RNA) PCR/qPCR, immunoassay, or enzymatic assay to detect host, as well as phage-based (plaque assay or phage amplification and lysis plus DNA, phage offspring, or intracellular elements) techniques. When compared to culture for food testing, several of these more recent techniques, notably phage-based techniques, offer promise in terms of spathe ed, sensitivity of detection, and cost. This study examines the existing limitations and potential future applications of these innovative technologies for food testing as well as their advantages and disadvantages for identifying live pathogens in food. Nafisa Rashid Naim Ul Haque Ahsan Sakib B. Biotechnology 2023-04-02T04:32:44Z 2023-04-02T04:32:44Z 2022 2022-09 Thesis ID: 18136031 ID: 18336011 ID: 18336022 http://hdl.handle.net/10361/18043 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 40 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Cell viability Detection methods Foodborne pathogens Rapid methods Viable-but-nonculturable. Vibrio infections |
| spellingShingle |
Cell viability Detection methods Foodborne pathogens Rapid methods Viable-but-nonculturable. Vibrio infections Rashid, Nafisa Ul Haque, Naim Sakib, Ahsan Rapid detection of viable Vibrio Cholerae by Bacteriophage |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. |
| author2 |
Bin Naser, Iftekhar |
| author_facet |
Bin Naser, Iftekhar Rashid, Nafisa Ul Haque, Naim Sakib, Ahsan |
| format |
Thesis |
| author |
Rashid, Nafisa Ul Haque, Naim Sakib, Ahsan |
| author_sort |
Rashid, Nafisa |
| title |
Rapid detection of viable Vibrio Cholerae by Bacteriophage |
| title_short |
Rapid detection of viable Vibrio Cholerae by Bacteriophage |
| title_full |
Rapid detection of viable Vibrio Cholerae by Bacteriophage |
| title_fullStr |
Rapid detection of viable Vibrio Cholerae by Bacteriophage |
| title_full_unstemmed |
Rapid detection of viable Vibrio Cholerae by Bacteriophage |
| title_sort |
rapid detection of viable vibrio cholerae by bacteriophage |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18043 |
| work_keys_str_mv |
AT rashidnafisa rapiddetectionofviablevibriocholeraebybacteriophage AT ulhaquenaim rapiddetectionofviablevibriocholeraebybacteriophage AT sakibahsan rapiddetectionofviablevibriocholeraebybacteriophage |
| _version_ |
1814307493193449472 |