A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2020.
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Brac University
2023
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| Acesso em linha: | http://hdl.handle.net/10361/18033 |
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10361-18033 |
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10361-180332023-03-28T21:01:52Z A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city Rafique, Afra Anika Sumaiya, Jannatul Monjurul Haque, Dr. Fahim Kabir Department of Mathematics and Natural Sciences, Brac University Microbial water quality Helicobacter Pylori Microbial growth Microbial contamination This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2020. Catalogued from PDF version of thesis. Includes bibliographical references (pages 28-29). Water is indispensable for human survival. According to the World Health Organization, around two billion people in the world drink fecal contaminated water. This means there can be higher chances for the presence of the Helicobacter pylori in drinking water. H. pylori infection is the most common cause for gastric ulcer. In this study, water samples were collected in sterile autoclaved bottles from different areas of Dhaka City. Different media were used to detect fecal coliform, total coliform and differentiated colonies of H. pylori. The identification of H. pylori was carried out by biochemical tests, and PCR followed by gel electrophoresis. A 520 base pair of H. pylori DNA was found in the result of gel electrophoresis. From the result of this study, it is found that the presence of fecal and total coliform is at an alarming rate in the underprivileged areas (Korail slum and Bossila slum) than privileged areas. H. pylori has also been successfully isolated from the water samples taken from Korail slum. Afra Anika Rafique Jannatul Sumaiya B. Biotechnology 2023-03-28T08:29:57Z 2023-03-28T08:29:57Z 2020 2020-08 Thesis ID: 15336017 ID: 16336008 http://hdl.handle.net/10361/18033 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 34 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Microbial water quality Helicobacter Pylori Microbial growth Microbial contamination |
| spellingShingle |
Microbial water quality Helicobacter Pylori Microbial growth Microbial contamination Rafique, Afra Anika Sumaiya, Jannatul A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2020. |
| author2 |
Monjurul Haque, Dr. Fahim Kabir |
| author_facet |
Monjurul Haque, Dr. Fahim Kabir Rafique, Afra Anika Sumaiya, Jannatul |
| format |
Thesis |
| author |
Rafique, Afra Anika Sumaiya, Jannatul |
| author_sort |
Rafique, Afra Anika |
| title |
A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city |
| title_short |
A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city |
| title_full |
A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city |
| title_fullStr |
A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city |
| title_full_unstemmed |
A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city |
| title_sort |
study of microbial water quality check and isolation of helicobacter pylori from water samples taken from dhaka city |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/18033 |
| work_keys_str_mv |
AT rafiqueafraanika astudyofmicrobialwaterqualitycheckandisolationofhelicobacterpylorifromwatersamplestakenfromdhakacity AT sumaiyajannatul astudyofmicrobialwaterqualitycheckandisolationofhelicobacterpylorifromwatersamplestakenfromdhakacity AT rafiqueafraanika studyofmicrobialwaterqualitycheckandisolationofhelicobacterpylorifromwatersamplestakenfromdhakacity AT sumaiyajannatul studyofmicrobialwaterqualitycheckandisolationofhelicobacterpylorifromwatersamplestakenfromdhakacity |
| _version_ |
1814307071908118528 |