Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
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Brac University
2023
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10361-179582023-03-22T04:53:59Z Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. Rafia, Zarin Tasnim Monjurul Haque, Dr. Fahim Kabir Department of Mathematics and Natural Sciences, Brac University Metallo-beta-lactamase Extended spectrum-beta-lactamase Carbapenem blaNDM1 Wastewater Antibiotics Microbiology Beta lactamases. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 17-20). Background: The co-existence of MBL and ESBL in Gram-negative bacteria poses greater risk in public health and limit the range of treatment choices, even with β-lactams which is the most widely used class of antibiotics. Untreated wastewater from hospitals increasing the opportunities to transfer the antibiotics resistance gene between pathogens which ultimately reach communities and poses health risks. Metallo-β-lactamase (MBL), that is one type of carbapenems enzyme, exhibit the ability to inactivate all classes of β-lactams antibiotics. With discovering of blaNDM-1 gene, a novel β-lactamase, produced the New Delhi Metallo- β-lactamase, poses a larger threat as this gene usually found along the side of other genes that provide resistance to almost all antibiotics. This blaNDM-1 gene is easily transferable between Enterobacteriaceae. ESBL is another commonly found enzyme of Enterobacteriaceae that inactivate cephalosporins and monobactams. Both these genes are important to be identified and prevention of their spread. Materials and Methods: Wastewater samples were collected from 18 sites in Dhaka and transferred to the laboratory. Bacterial cultures were grown on selective agar media for isolation of Klebsiella pneumoniae. Antibiotic resistance profiles of colonies were determined with Kirby Bauer disc diffusion testing with PCR identification of MBL encoding genes, blaNDM-1, and blaIMP, as well as ESBL encoding genes, blaSHV, blaTEM, and blaCTX-M. Results: From this study it can be perceived that, 29.88% of Klebsiella pneumoniae which were either imipenem or meropenem resistant or both of antibiotic resistant, most of them carried the blaNDM-1 or blaSHV gene which is the most common gene responsible for coding the MBL & ESBL enzyme known as Metallo-beta-lactamase that makes bacteria resistant to a broad range of beta lactam antibiotics. Zarin Tasnim Rafia B. Microbiology 2023-03-13T05:56:19Z 2023-03-13T05:56:19Z 2022 2022-09 Thesis ID: 18126002 http://hdl.handle.net/10361/17958 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 20 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Metallo-beta-lactamase Extended spectrum-beta-lactamase Carbapenem blaNDM1 Wastewater Antibiotics Microbiology Beta lactamases. |
| spellingShingle |
Metallo-beta-lactamase Extended spectrum-beta-lactamase Carbapenem blaNDM1 Wastewater Antibiotics Microbiology Beta lactamases. Rafia, Zarin Tasnim Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. |
| author2 |
Monjurul Haque, Dr. Fahim Kabir |
| author_facet |
Monjurul Haque, Dr. Fahim Kabir Rafia, Zarin Tasnim |
| format |
Thesis |
| author |
Rafia, Zarin Tasnim |
| author_sort |
Rafia, Zarin Tasnim |
| title |
Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. |
| title_short |
Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. |
| title_full |
Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. |
| title_fullStr |
Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. |
| title_full_unstemmed |
Characterizing the Co-Existence of Metallo-β-lactamase and Extended Spectrum Beta-Lactamase genes in Klebsiella pneumoniae isolates in community wastewater samples of Dhaka, Bangladesh. |
| title_sort |
characterizing the co-existence of metallo-β-lactamase and extended spectrum beta-lactamase genes in klebsiella pneumoniae isolates in community wastewater samples of dhaka, bangladesh. |
| publisher |
Brac University |
| publishDate |
2023 |
| url |
http://hdl.handle.net/10361/17958 |
| work_keys_str_mv |
AT rafiazarintasnim characterizingthecoexistenceofmetalloblactamaseandextendedspectrumbetalactamasegenesinklebsiellapneumoniaeisolatesincommunitywastewatersamplesofdhakabangladesh |
| _version_ |
1814309792608419840 |