Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
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| التنسيق: | أطروحة |
| اللغة: | en_US |
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Brac University
2022
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/17567 |
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10361-17567 |
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10361-175672022-11-15T21:01:42Z Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South Raymond, Mugenyi Precious, Bisoboka Claire Haque, Ashrin Rahman, Mohammad Mushfiqur Siddiqee, Mahbubul Hasan Department of Mathematics and Natural Sciences, Brac University Carbapenem-Resistant Enterobacteriaceae bla-NDM1 bla-OXA2 Klebsiella pneumoniae Hospital Adjacent Area Bacterial diseases. Pneumonia. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 64-77). Carbapenems are commonly considered last-resort antibiotics in the treatment of severe infections caused by multidrug-resistant Enterobacteriaceae. The emergence of carbapenem-resistant isolates over the last decade has been a major concern as it has contributed to narrowing the current treatment palette, leaving few or in some cases optimal treatment options. The present research was done to isolate and assess the prevalence of carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples from hospital adjacent areas in Dhaka South. In this study, 55 vegetable and 50 water samples were randomly collected from different local markets near hospitals in Dhaka South. These samples were collected from September 2021 to November 2021. Out of 606 strains isolated throughout the study (from both enrichment and without enrichment cultures), 77 (12.71%) were Klebsiella pneumoniae. These isolates were subjected to an antibiotic susceptibility test using the following 12 antibiotics; Meropenem+EDTA (MR+ED), Imipenem (IMI10), Kanamycin(K30), Gentamicin (CN10), Ceftazidime (CAZ30), Erythromycin(E15), Nitrofurantoin (NIT300), Ampicillin (AMP10), Amoxyclav (AMC30), Nalidixic acid (NA30), Streptomycin(S10), and Tetracycline (TE30). All the 77 Klebsiella pneumoniae isolates were found to be resistant to Meropenem + EDTA (100%), followed by Nalidix Acid and Erythromycin to which 72.70% and 63.60% were resistant, respectively. This was then followed by Kanamycin (57.10%), Ampicillin (55.80%), Ceftazidime (51.90%), Nitrofurantoin (49.40%), Tetracycline (33.80%), Streptomycin (18.20%), Imipenem (15.60%), Gentamicin (10.40%) and Amoxyclav (7.80%). 4 (5.19%) of the K. pneumoniae isolates were resistant to all the 12 tested antibiotics. When the Multiple Antibiotic Resistance (MAR) index was calculated, 5(6.49%), 39(50.65%), 29(37.66%), and 4(5.19%) were identified under MAR indices <0.2, 0.2-<0.5, 0.5-<1 and 1 respectively. PCR was used to detect carbapenemase genes; blaNDM-1 and blaOXA-2 in 59 DNA-extracted K. pneumoniae isolates. 5(8.47%) of the isolates were positive for the blaNDM-1 gene, out of which, 3 and 2 came from samples collected from Anandabazar and Moulovibazar respectively. On the other hand, 9(15.25%) of the isolates were positive for the blaOXA-2 gene, out of which, 5, 3, and 1 came from samples collected from Anandabazar, Moulovibazar, and Moghbazar respectively. The findings of the current study demonstrated the potential of vegetables and water from markets located in hospital adjacent areas of Dhaka South, to be reservoirs of carbapenem-resistant - multidrug-resistant bacteria Mugenyi Raymond Bisoboka Claire Precious Ashrin Haque Mohammad Mushfiqur Rahman B. Microbiology 2022-11-15T05:15:01Z 2022-11-15T05:15:01Z 2022 2022-03 Thesis ID: 16226013 ID: 16236012 ID: 17126030 ID: 17126032 http://hdl.handle.net/10361/17567 en_US Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 77 Pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
en_US |
| topic |
Carbapenem-Resistant Enterobacteriaceae bla-NDM1 bla-OXA2 Klebsiella pneumoniae Hospital Adjacent Area Bacterial diseases. Pneumonia. |
| spellingShingle |
Carbapenem-Resistant Enterobacteriaceae bla-NDM1 bla-OXA2 Klebsiella pneumoniae Hospital Adjacent Area Bacterial diseases. Pneumonia. Raymond, Mugenyi Precious, Bisoboka Claire Haque, Ashrin Rahman, Mohammad Mushfiqur Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. |
| author2 |
Siddiqee, Mahbubul Hasan |
| author_facet |
Siddiqee, Mahbubul Hasan Raymond, Mugenyi Precious, Bisoboka Claire Haque, Ashrin Rahman, Mohammad Mushfiqur |
| format |
Thesis |
| author |
Raymond, Mugenyi Precious, Bisoboka Claire Haque, Ashrin Rahman, Mohammad Mushfiqur |
| author_sort |
Raymond, Mugenyi |
| title |
Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South |
| title_short |
Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South |
| title_full |
Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South |
| title_fullStr |
Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South |
| title_full_unstemmed |
Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South |
| title_sort |
prevalence of carbapenem-resistant klebsiella pneumoniae in vegetable and water samples in dhaka south |
| publisher |
Brac University |
| publishDate |
2022 |
| url |
http://hdl.handle.net/10361/17567 |
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| _version_ |
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