Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
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| التنسيق: | أطروحة |
| اللغة: | en_US |
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Brac University
2022
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/17531 |
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10361-175312022-10-24T21:01:37Z Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City Bulbuli, Naima Hasan Awysharjaw, Rownak Showkat Lohani, Tabassum Md. Hasanuzzaman Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Pathogens Antibiotic Dhaka Hospital wastewater Community wastewater Multi Drug Resistance Medical wastes Medical centers--Waste disposal This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 31-32). Background: Uncontrolled and excessive exposure of antibiotics in the medical waste poses a selective pressure to acquire and disseminate highly antibiotic resistance genes to common pathogenic bacteria. This study is aimed to isolate common pathogenic bacteria and prevalence of multiple drug resistance genes in community and hospital wastewater. Materials and Methods: Ten different wastewater samples (5 hospitals and 5 community wastewater) were aseptically collected and bacteriological investigations were performed for quantification and identification of bacterial isolates. Antibiotic susceptibility was performed by the Kirby-Bauer disk diffusion method and interpreted using the CLSI guideline. Polymerase chain-reaction (PCR) will be performed for identification of ESBL and carbapenem resistance genes. Results: Out of 234 isolates collected (based on different morphology); Gram-negative bacteria were predominant (85.04%). Top pathogenic-bacteria isolates were E.coli-(15%); Klebsiella pneumonia (12.82%); Pseudomonas aeruginosa (8.11%); Salmonella sp. (7.26%); Shigella sp. (6.84%); Staphylococcus aureus (6%). Overall 76.92% isolates were resistant to ampicillin and 34.61%, 40%, 23.08% and 12.39% were resistant to third-generation cephalosporin, vancomycin, colistin and carbapenem respectively. From overall isolates, the MDR and XDR organisms were found to be 84.62% and 5.13% respectively. PCR data on ESBL and carbapenem resistance genes are ongoing. Conclusion: High prevalence of multiple-drug-resistance bacteria in both hospital and community wastewater is indicating the dissemination of these pathogens in community which may lead to great public health issues if the situation is not tackled in near future. Naima Hasan Bulbuli Rownak Showkat Awysharjaw Tabassum Lohani B. Microbiology 2022-10-24T10:15:09Z 2022-10-24T10:15:09Z 2022 2022-05 Thesis ID: 18126058 ID: 18126057 ID: 18126015 http://hdl.handle.net/10361/17531 en_US Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 32 Pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
en_US |
| topic |
Pathogens Antibiotic Dhaka Hospital wastewater Community wastewater Multi Drug Resistance Medical wastes Medical centers--Waste disposal |
| spellingShingle |
Pathogens Antibiotic Dhaka Hospital wastewater Community wastewater Multi Drug Resistance Medical wastes Medical centers--Waste disposal Bulbuli, Naima Hasan Awysharjaw, Rownak Showkat Lohani, Tabassum Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. |
| author2 |
Md. Hasanuzzaman |
| author_facet |
Md. Hasanuzzaman Bulbuli, Naima Hasan Awysharjaw, Rownak Showkat Lohani, Tabassum |
| format |
Thesis |
| author |
Bulbuli, Naima Hasan Awysharjaw, Rownak Showkat Lohani, Tabassum |
| author_sort |
Bulbuli, Naima Hasan |
| title |
Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City |
| title_short |
Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City |
| title_full |
Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City |
| title_fullStr |
Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City |
| title_full_unstemmed |
Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City |
| title_sort |
prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in dhaka city |
| publisher |
Brac University |
| publishDate |
2022 |
| url |
http://hdl.handle.net/10361/17531 |
| work_keys_str_mv |
AT bulbulinaimahasan prevalenceanddiversityofantimicrobialresistantpathogensfrommedicalandcommunitywastewaterindhakacity AT awysharjawrownakshowkat prevalenceanddiversityofantimicrobialresistantpathogensfrommedicalandcommunitywastewaterindhakacity AT lohanitabassum prevalenceanddiversityofantimicrobialresistantpathogensfrommedicalandcommunitywastewaterindhakacity |
| _version_ |
1814307491983392768 |