Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2021.
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Brac University
2022
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10361-163472022-02-27T21:01:39Z Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains Tabassum, Tahani Shahriar, Sagarika Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Cas1 Cas3 Phylogenetic tree SNP Protein functional domains Cholera. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2021. Catalogued from PDF version of thesis. Includes bibliographical references (pages 60-62). Cholera is an extremely virulent waterborne disease caused by the ingestion of food or water contaminated with pathogenic strains of Vibrio cholerae. On the other hand, pathogenic strains of E. coli, particularly Shiga-toxin producing E. coli, are most commonly responsible for diarrheagenic illness, urinary tract infections, as well as life-threatening complications such as Hemolytic Uremic Syndrome (HUS). The presence of the CRISPR-Cas system in both of the bacterial species have raised major global concerns regarding the enhanced chances of pathogenicity or virulence in the bacterial strains due to this adaptive immune system. Besides, the presence of the CRISPR-Cas system within these species can interfere with the emerging phage therapy treatment approaches against drug-resistant bacteria. In this study, we aimed to assess the diversity of cas1 and cas3 protein sequences in the CRISPR locus of several CRISPR confirmed V. cholerae and E. coli strains, and characterize this diversity across the functional domains of the reference cas proteins. Moreover, we established the interspecies relatedness of both species in terms of their cas1 and cas3 sequences. Tahani Tabassum Sagarika Shahriar B. Biotechnology 2022-02-27T05:10:07Z 2022-02-27T05:10:07Z 2021 2021-12 Thesis ID 18136067 ID 18136087 http://hdl.handle.net/10361/16347 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 62 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Cas1 Cas3 Phylogenetic tree SNP Protein functional domains Cholera. |
| spellingShingle |
Cas1 Cas3 Phylogenetic tree SNP Protein functional domains Cholera. Tabassum, Tahani Shahriar, Sagarika Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2021. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Tabassum, Tahani Shahriar, Sagarika |
| format |
Thesis |
| author |
Tabassum, Tahani Shahriar, Sagarika |
| author_sort |
Tabassum, Tahani |
| title |
Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains |
| title_short |
Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains |
| title_full |
Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains |
| title_fullStr |
Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains |
| title_full_unstemmed |
Assessing the interspecies and intraspecies diversity of Cas1 and Cas3 proteins of the CRISPR-Cas system in vibrio cholera and Escherichia coli strains |
| title_sort |
assessing the interspecies and intraspecies diversity of cas1 and cas3 proteins of the crispr-cas system in vibrio cholera and escherichia coli strains |
| publisher |
Brac University |
| publishDate |
2022 |
| url |
http://hdl.handle.net/10361/16347 |
| work_keys_str_mv |
AT tabassumtahani assessingtheinterspeciesandintraspeciesdiversityofcas1andcas3proteinsofthecrisprcassysteminvibriocholeraandescherichiacolistrains AT shahriarsagarika assessingtheinterspeciesandintraspeciesdiversityofcas1andcas3proteinsofthecrisprcassysteminvibriocholeraandescherichiacolistrains |
| _version_ |
1814309205836824576 |