Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population
This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2010
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BRAC University
2012
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10361-16242019-09-30T04:22:02Z Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population Paul, Debashish Choudhury, Naiyyum Ali, Liaquat Department of Mathematical and Natural Science, BRAC University Biotechnology This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2010 Cataloged from PDF version of thesis report. Includes bibliographical references (page 63-79). Vitamin D has an important immunomodulatory property and also has been suggested to play an important role in cellular metabolism. Vitamin D receptor (VDR) gene polymorphism has also been found to be associated with insulin secretory capacity and glucose intolerance. The present study was undertaken to determine genotype of VDR gene common variants in young onset diabetic subjects of Bangladesh to explore its association with diabetes and B cell secretory capacity. A total number of 94 young diabetic subjects and 92 healthy controls were recruited. Detailed clinical and anthropometric measurements were recorded. Blood glucose was measured by glucoseoxidase, lipids by standard methods. C-peptide was estimated by enzyme linked immunosorbent assay (ELISA), and insulin secretory capacity (HOMA%B), sensitivity (HOMA%S), resistance (HOMA-IR) were calculated using VIOMA Sigma software. DNA was extracted using QIAGEN Blood DNA Kit. VDR gene variants [G>T and T>C] were determined by PCR-RFLP using restriction endonuclease Apal and Taq) respectively. Data were managed using Statistical Program for Social Science (SPSS). Unpaired Student's -`t' test and Chi-squared tests, as appropriate, were performed. Cpeptide level was found to be significantly lower 3.297 (p<0.001) and I-IOMA%B found lower 3.102 (p=0.003). The G>T and T>C genome frequencies (wild, heterozygous and homozygous variants) were in the control (0.183, 0.5 16, 0.3 16 and 0.370, 0.522, 0.109 respectively) and (0.198, 0.484, 0.319 and 0.484, 0.418, 0.090 respectively) in YDM which did not show significant association with YDM (p=0.906 and 0.288 respectively). Genotype frequency of the marker allele did not show significant association with glucose, C-peptide, 1IOMA%S and IR either in Controls or YDM subjects except the HOMA%B shown to have relatively lower. Debashish Paul M. Biotechnology 2012-03-01T06:56:23Z 2012-03-01T06:56:23Z 2010 2010-04 Thesis ID 08376001 http://hdl.handle.net/10361/1624 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 83 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biotechnology |
| spellingShingle |
Biotechnology Paul, Debashish Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2010 |
| author2 |
Choudhury, Naiyyum |
| author_facet |
Choudhury, Naiyyum Paul, Debashish |
| format |
Thesis |
| author |
Paul, Debashish |
| author_sort |
Paul, Debashish |
| title |
Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population |
| title_short |
Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population |
| title_full |
Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population |
| title_fullStr |
Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population |
| title_full_unstemmed |
Association of vitamin D receptor gene polymorphism with young onset diabetes millitus in a Bangladeshi population |
| title_sort |
association of vitamin d receptor gene polymorphism with young onset diabetes millitus in a bangladeshi population |
| publisher |
BRAC University |
| publishDate |
2012 |
| url |
http://hdl.handle.net/10361/1624 |
| work_keys_str_mv |
AT pauldebashish associationofvitamindreceptorgenepolymorphismwithyoungonsetdiabetesmillitusinabangladeshipopulation |
| _version_ |
1814309205501280256 |