Biased acquisition of spacers by the CRISPR System of the V. cholerae
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2021.
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| التنسيق: | أطروحة |
| اللغة: | English |
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Brac University
2021
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/15564 |
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10361-15564 |
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10361-155642021-10-27T21:01:39Z Biased acquisition of spacers by the CRISPR System of the V. cholerae Supan, Erena Tuzneen Haque, Nayeema Momtaz, Monira Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Vibrio cholerae Bacteriophage CRISPR Spacer PLE Cholera. Bacterial genetics. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2021. Catalogued from PDF version of thesis. Includes bibliographical references (pages 40-43). Cholera is a deadly waterborne disease that is caused by the serogroup of O1 or O139 of Vibrio cholerae. Also, there are many non-pathogenic Vibrio cholerae strains known as non-O1 and non-O139. Bacteriophages are bacteria eating viruses and are highly specific towards the host cell. They require host cellular machinery to perform their metabolic activities. Many of the Vibrio cholerae strains contain CRISPR which is an immune defense system that targets foreign DNA like plasmids, phage DNA and even other bacterial DNA that are inserted into CRISPR arrays. When a phage or any invader attacks bacteria, the CRISPR system inserts the fragments of phage or the invader’s DNA into the CRISPR array. It is known as spacers which later work as an immune memory. This study includes analysis of V. cholerae strains that contain CRISPR, specifically the in depth analysis of spacers using different bioinformatics tools and softwares. One of the major findings of this study was that the spacers come from some specific regions of the phage whole genome. Erena Tuzneen Nayeema Haque Monira Momtaz B. Biotechnology 2021-10-27T05:50:11Z 2021-10-27T05:50:11Z 2021 2021-09 Thesis ID 17136010 ID 17136028 ID 17136005 http://hdl.handle.net/10361/15564 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 43 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Vibrio cholerae Bacteriophage CRISPR Spacer PLE Cholera. Bacterial genetics. |
| spellingShingle |
Vibrio cholerae Bacteriophage CRISPR Spacer PLE Cholera. Bacterial genetics. Supan, Erena Tuzneen Haque, Nayeema Momtaz, Monira Biased acquisition of spacers by the CRISPR System of the V. cholerae |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2021. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Supan, Erena Tuzneen Haque, Nayeema Momtaz, Monira |
| format |
Thesis |
| author |
Supan, Erena Tuzneen Haque, Nayeema Momtaz, Monira |
| author_sort |
Supan, Erena Tuzneen |
| title |
Biased acquisition of spacers by the CRISPR System of the V. cholerae |
| title_short |
Biased acquisition of spacers by the CRISPR System of the V. cholerae |
| title_full |
Biased acquisition of spacers by the CRISPR System of the V. cholerae |
| title_fullStr |
Biased acquisition of spacers by the CRISPR System of the V. cholerae |
| title_full_unstemmed |
Biased acquisition of spacers by the CRISPR System of the V. cholerae |
| title_sort |
biased acquisition of spacers by the crispr system of the v. cholerae |
| publisher |
Brac University |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10361/15564 |
| work_keys_str_mv |
AT supanerenatuzneen biasedacquisitionofspacersbythecrisprsystemofthevcholerae AT haquenayeema biasedacquisitionofspacersbythecrisprsystemofthevcholerae AT momtazmonira biasedacquisitionofspacersbythecrisprsystemofthevcholerae |
| _version_ |
1814308754910347264 |