Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2020.
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Brac University
2021
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| Sarrera elektronikoa: | http://hdl.handle.net/10361/15510 |
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10361-155102021-10-21T21:01:24Z Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios Upoma, Shifat Sehrin Khan Akter, Raushanara Department of Pharmacy, Brac University Antioxidant DPPH Cytotoxicity N4X4 Triphala Antioxidants--Physiological effect This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2020. Cataloged from PDF version of thesis report. Includes bibliographical references (pages 58-67). Triphala is comprised of Bibhitaki Amalaki and Haritaki and its methanol extract was chosen for the In vitro antioxidant and cytotoxic potential studies. Firstly, in the DPPH test the IC50 value was found to be 15.93 μg/ mL, 12.99 μg/ mL and 10.287 μg/ mL for Haritaki, Bibhitaki and Amlaki, and the IC50 value for Ascorbic Acid was 12.96 μg/ mL, so Amlaki showed more inhibition. The IC50 value for the Triphala ratios 1:1:1 (A:B:H), 2:1:1 (A:B:H), 1:2:1 (A:B:H) and 1:1:2 (A:B:H) were 11.28 μg/ mL, 7.01 μg/ mL, 8.33 μg/ mL and 11.03 μg/ mL respectively and all the values were lower than that of the standard. Secondly, at 1200 μg/ mL, the total phenolic content was found to be 368.94 mg, 395.09 mg and 260.65 mg for Bibhitaki, Amlaki and Haritaki respectively. Finally, at 1200 μg/ mL, the total antioxidant capacity was found to be 245.32mg, 485.5mg and 559.76mg for Bibhitaki, Amlaki and Haritaki respectively. In N4X4 cell line, IC50 value was 13.43mg/ mL and 13.23mg/ mL at 25 mg/ mL for 1:1:1(A:B:H) and 2:1:1 (A:B:H) Triphala ratio. To conclude, it can be inferred that the extracts showed strong antioxidant and moderate cytotoxic potential. Shifat Sehrin Khan Upoma B. Pharmacy 2021-10-21T05:59:03Z 2021-10-21T05:59:03Z 2020 2020-02 Thesis ID 16146036 http://hdl.handle.net/10361/15510 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 67 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Antioxidant DPPH Cytotoxicity N4X4 Triphala Antioxidants--Physiological effect |
| spellingShingle |
Antioxidant DPPH Cytotoxicity N4X4 Triphala Antioxidants--Physiological effect Upoma, Shifat Sehrin Khan Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2020. |
| author2 |
Akter, Raushanara |
| author_facet |
Akter, Raushanara Upoma, Shifat Sehrin Khan |
| format |
Thesis |
| author |
Upoma, Shifat Sehrin Khan |
| author_sort |
Upoma, Shifat Sehrin Khan |
| title |
Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios |
| title_short |
Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios |
| title_full |
Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios |
| title_fullStr |
Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios |
| title_full_unstemmed |
Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios |
| title_sort |
investigation of in vitro antioxidant and cytotoxic potential of triphala methanol extract in different ratios |
| publisher |
Brac University |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10361/15510 |
| work_keys_str_mv |
AT upomashifatsehrinkhan investigationofinvitroantioxidantandcytotoxicpotentialoftriphalamethanolextractindifferentratios |
| _version_ |
1814309203114721280 |