Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana

Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana

This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2021.

Dades bibliogràfiques
Autor principal: Nowrid, Nazia
Altres autors: Mubassir, M H M
Format: Thesis
Idioma:English
Publicat: Brac University 2021
Matèries:
Pattern-triggered immunity (PTI)
PRR FLS2
BAK1
Arabidopsis thaliana
Immunity
Accés en línia:http://hdl.handle.net/10361/14948
id 10361-14948
record_format dspace
spelling 10361-149482021-08-08T21:01:19Z Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana Nowrid, Nazia Mubassir, M H M Department of Mathematics and Natural Sciences, Brac University Pattern-triggered immunity (PTI) PRR FLS2 BAK1 Arabidopsis thaliana Immunity This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2021. Catalogued from PDF version of thesis. Includes bibliographical references (pages 46-54). Pattern-triggered immunity (PTI) acts as the first layer of defense that is deployed by plants in order to avert microbial invasions. It is actually identified by the activities of patternrecognition receptors (PRRs), that bind with the pathogen-associated molecular patterns (PAMPs) and recruit co-receptor protein(s) to convey a defense signal, thereby initiating the plant’s immunity. In this study, the 122nd amino acid in BAK1 from the crystallographic structure of FLS2-BAK1-flg22 complex (PDB ID: 4MN8) was mutated from aspartate to asparagine using in-silico method. Molecular dynamics simulations (100 ns) and MM/PBSA calculations were then applied for a thorough and comprehensive analysis of the interactions between PRR FLS2, mutated co-receptor mBAK1 and PAMP flg22. ARG-72 and LYS-36 from FLS2-mBAK1 (in the presence and absence of flg22 respectively), ARG-72 and GLU- 28 from mBAK1-flg22 (in the presence and absence of FLS2 respectively) & LYS-77 and ASP-176 from FLS2-flg22 (in the presence and absence of mBAK1 respectively) were revealed to be the most prominent residues, aiding notably in the process of heterodimerization during PTI, subsequently mediated by FLS2. A hypothesis can thereby be established, that mutation at any of these residues will affect the PTI of Arabidopsis thaliana. The simulations were also compared using parameters such as root mean square deviation (RMSD), root mean square fluctuation (RMSF), the radius of gyration (Rg), solvent accessible surface areas (SASA) and the number of hydrogen bonds (H-bond) to comprehend the structural integrity of the complex. The obtained results demonstrated that PAMP flg22 interacted much more favorably with the PRR FLS2 in the presence of mutated co-receptor mBAK1 in the complex, which implicates the necessity of the co-receptor in FLS2 mediated PTI to recognize PAMP flg22. Furthermore, since FLS2 has been shown to play a key role in Arabidopsis thaliana plant defense mechanism, its assumed binding mechanism with PAMP and co-receptor BAK1 will help paint an improved comprehension of the inceptive stages of PTI. Nazia Nowrid M. Biotechnology 2021-08-08T09:38:22Z 2021-08-08T09:38:22Z 2021 2021-03 Thesis ID 18376004 http://hdl.handle.net/10361/14948 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 54 pages application/pdf Brac University
institution Brac University
collection Institutional Repository
language English
topic Pattern-triggered immunity (PTI)
PRR FLS2
BAK1
Arabidopsis thaliana
Immunity
spellingShingle Pattern-triggered immunity (PTI)
PRR FLS2
BAK1
Arabidopsis thaliana
Immunity
Nowrid, Nazia
Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana
description This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2021.
author2 Mubassir, M H M
author_facet Mubassir, M H M
Nowrid, Nazia
format Thesis
author Nowrid, Nazia
author_sort Nowrid, Nazia
title Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana
title_short Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana
title_full Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana
title_fullStr Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana
title_full_unstemmed Structural dynamics of PRR FLS2, PAMP flg22 and mutated co-receptor BAK1 of Arabidopsis thaliana
title_sort structural dynamics of prr fls2, pamp flg22 and mutated co-receptor bak1 of arabidopsis thaliana
publisher Brac University
publishDate 2021
url http://hdl.handle.net/10361/14948
work_keys_str_mv AT nowridnazia structuraldynamicsofprrfls2pampflg22andmutatedcoreceptorbak1ofarabidopsisthaliana
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