Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge

This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2020.

Detalles Bibliográficos
Autor principal: Sultana, Sumaiya
Otros Autores: Siddique, Romana
Formato: Tesis
Lenguaje:en_US
Publicado: Brac University 2021
Materias:
Acceso en línea:http://hdl.handle.net/10361/14741
id 10361-14741
record_format dspace
spelling 10361-147412021-07-07T05:48:45Z Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge Sultana, Sumaiya Siddique, Romana Department of Mathematics and Natural Sciences, Brac University Reactive dye Dye degrading bacteria Textile effluent Decolorization rate Salt Media (SM) Spectrophotometry This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2020. Catalogued from PDF version of thesis. Includes bibliographical references (pages 35-40). Textile industries use various dyes which have become one of the key sources of environment pollution. As a result, it is necessary to remove these undesirable dyes from the effluent that is released to the environment. This study was envisaged to obtain potential bacterium that can degrade the reactive dyes present in soil/sludge samples collected from the textile disposal sites. Samples were collected aseptically from Narsingdi, Bangladesh. On the basis of the degradation rate, three bacterial strains were isolated using salt media (SM) for two dyes named Setazol Blue BB and Black B. Isolated bacterial strains were identified as Bacillus sp. (SSR_S4_19, SSR_B-3_19), Pseudomonas sp. (SSR_S-5_19) by 16srRNA gene sequencing analysis. For the decolorization experiment, SM broth containing specific dyes (1%) were inoculated with each of the strains. All the three strains could decolorize the soil sample within 24h which was estimated using spectrophotometry. The decolorization rate for Setazol Blue BB was 87.7% and 86.5% in 24hr for the strains SSR_S-4_19 and SSR_S-5_19 respectively. On the other hand, for Black B it was 92.6% and 85.22% in 24hr for the strains SSR_B-3_19 and SSR_S-4_19 respectively. Since these strains have been efficient in decolorizing the dyes, in future it may be a promising strain for industrial use. Sumaiya Sultana B. Microbiology 2021-07-06T06:45:53Z 2021-07-06T06:45:53Z 2020 2020-02 Thesis ID: 15226004 http://hdl.handle.net/10361/14741 en_US Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 40 Pages application/pdf Brac University
institution Brac University
collection Institutional Repository
language en_US
topic Reactive dye
Dye degrading bacteria
Textile effluent
Decolorization rate
Salt Media (SM)
Spectrophotometry
spellingShingle Reactive dye
Dye degrading bacteria
Textile effluent
Decolorization rate
Salt Media (SM)
Spectrophotometry
Sultana, Sumaiya
Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
description This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2020.
author2 Siddique, Romana
author_facet Siddique, Romana
Sultana, Sumaiya
format Thesis
author Sultana, Sumaiya
author_sort Sultana, Sumaiya
title Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
title_short Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
title_full Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
title_fullStr Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
title_full_unstemmed Isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
title_sort isolation, characterization and phylogenetic analysis of dye decolorizing bacteria from textile sludge
publisher Brac University
publishDate 2021
url http://hdl.handle.net/10361/14741
work_keys_str_mv AT sultanasumaiya isolationcharacterizationandphylogeneticanalysisofdyedecolorizingbacteriafromtextilesludge
_version_ 1814307062129098752