Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2020.
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Brac University
2021
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10361-144712021-07-07T05:15:41Z Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools Arthi, Safwana Murshed Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Vibrio cholerae Bacteriophages Cholera Pandemic Genome Sequencing This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2020. Catalogued from PDF version of thesis. Includes bibliographical references (pages 30-31). Vibrio cholerae is an opportunistic, gram-negative bacterium that is commonly found in fresh and saltwater, soil, food, or in the intestine of humans and animals. It is the most causative agent of cholera; a chronic diarrheal disease with epidemic and pandemic potentials that might lead to death if untreated. Cholera has been recognized as a significant public health problem around the world and already faced seven cholera pandemics. Lytic bacteriophages specific to V. cholerae are the most potent triggers of causing cholera outbreaks by killing bacteria present in the infected individuals and environmental reservoir. This study includes molecular isolation of bacteriophage DNA from the environmental water samples and bioinformatics software analysis as well. However, a very unique bacteriophage (WT1774), was isolated from this study which showed the least similarities among other phages. Although the whole genome sequencing might not be done properly, all relevant computational analyses (including genomic and phenotypic characteristics) couldn’t be performed as expected. To retrieve all essential information for higher research, further analysis is needed. Safwana Murshed Arthi B. Biotechnology 2021-06-03T05:20:48Z 2021-06-03T05:20:48Z 2020-08 Thesis ID 15336009 http://hdl.handle.net/10361/14471 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 31 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Vibrio cholerae Bacteriophages Cholera Pandemic Genome Sequencing |
| spellingShingle |
Vibrio cholerae Bacteriophages Cholera Pandemic Genome Sequencing Arthi, Safwana Murshed Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2020. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Arthi, Safwana Murshed |
| format |
Thesis |
| author |
Arthi, Safwana Murshed |
| author_sort |
Arthi, Safwana Murshed |
| title |
Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| title_short |
Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| title_full |
Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| title_fullStr |
Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| title_full_unstemmed |
Isolation of Lytic Bacteriophages from Environmental water samples specific to Vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| title_sort |
isolation of lytic bacteriophages from environmental water samples specific to vibrio cholerae; software-based analysis of whole-genome using bioinformatics tools |
| publisher |
Brac University |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10361/14471 |
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AT arthisafwanamurshed isolationoflyticbacteriophagesfromenvironmentalwatersamplesspecifictovibriocholeraesoftwarebasedanalysisofwholegenomeusingbioinformaticstools |
| _version_ |
1814308752664297472 |