Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2020.
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Brac University
2021
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| Accès en ligne: | http://hdl.handle.net/10361/14386 |
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10361-143862021-03-24T21:01:28Z Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities Kabir, A.B.M. Rezwanul Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Bacteriophage Propagation Host cell density Vibrio spp. Escherichia coli This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2020. Catalogued from PDF version of thesis. Includes bibliographical references (pages 46-51). It has been reported in vitro that bacteriophages require a certain host cell density in order to propagate. However, previous studies have shown that in environment, especially in water bodies, bacteriophage infection takes place even when host cell densities are lower than the required threshold density observed in vitro. This leads to the indication that some factors must be in play that allows bacteriophages to propagate at such low host cell densities in the environment. In this study we investigated the effect of host cell densities on bacteriophage propagation in phage-host systems of Vibrio spp. and Shiga toxin-producing Escherichia coli (STEC) and determined the respective threshold densities. We found that phage propagation ceased at host cell densities beyond the threshold density but did initiate when supplemented with spent media of the respective phage and host cultures at the logarithmic phase of infection. Hence, we hypothesize that molecules produced by the host and/or phage have the ability to influence how bacteriophages take decision to infect its host cell, especially in the environment. Although we investigated the effect of these molecules in the phage-host system of Vibrio spp. only, similar studies with phage-host systems of different species may provide valuable insights to our understanding of phage-host interaction and therefore may contribute to the field of phage therapy. A.B.M. Rezwanul Kabir M. Biotechnology 2021-03-24T09:33:59Z 2021-03-24T09:33:59Z 2020 2020-06 Thesis ID: 18376010 http://hdl.handle.net/10361/14386 en_US Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 52 pages application/pdf Brac University |
| institution |
Brac University |
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Institutional Repository |
| language |
en_US |
| topic |
Bacteriophage Propagation Host cell density Vibrio spp. Escherichia coli |
| spellingShingle |
Bacteriophage Propagation Host cell density Vibrio spp. Escherichia coli Kabir, A.B.M. Rezwanul Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2020. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Kabir, A.B.M. Rezwanul |
| format |
Thesis |
| author |
Kabir, A.B.M. Rezwanul |
| author_sort |
Kabir, A.B.M. Rezwanul |
| title |
Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| title_short |
Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| title_full |
Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| title_fullStr |
Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| title_full_unstemmed |
Can phages be cheated? - A study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| title_sort |
can phages be cheated? - a study on the influence of inducing molecules on bacteriophage propagation at low host cell densities |
| publisher |
Brac University |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10361/14386 |
| work_keys_str_mv |
AT kabirabmrezwanul canphagesbecheatedastudyontheinfluenceofinducingmoleculesonbacteriophagepropagationatlowhostcelldensities |
| _version_ |
1814309425103503360 |