Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract
This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2019.
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Brac University
2020
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10361-139072020-08-06T21:01:22Z Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract Sultana, Sharmin Hossain, Mahboob Ahsan, Chowdhury Rafiqul Department of Mathematics and Natural Sciences, Brac University Kupchan method Antioxidant activity Anticancer activity Hemotological parameters Thin layer chromatography This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2019. Catalogued from PDF version of thesis. Includes bibliographical references (pages 118-130). Mangrove ecosystems have been known to possess diverse secondary metabolites and a good number of bioactive compounds of pharmaceutical importance have been reported from the mangrove plants. Mangrove plants are biochemically unique, producing wide array of natural products with unique bioactivity. This study was undertaken to evaluate the in vitro antioxidant and in vivo anticancer activity of Excoecaria agallocha (bark) which is a mangrove plant. E. agallocha possess various therapeutically important properties and their different parts have been experimented for antioxidant and anticancer activities in various studies. The objective of this study was to evaluate the bark extract of E. agallocha and to find their antioxidant content and also in vivo anticanceractivity in mice model. For this purpose, dried powdered bark of E. agallocha was extracted by kupchan method of solvent-solvent partitioning and after that polarity band was observed by Thin layer of Chromatography (TLC). In thein vitro antioxidant activity test, the extracts were tested for CUPRAC reducing capacity, total antioxidant capacity, total phenolic content and total flavonoid content. Where, Methanolic bark extract showed highest antioxidant content than other solvent extracts. After that anticancer activity was done in mice model, where comparison of the body weight among the groups of mice and hematological parameters was measured by analyzing clinical tests. In sub-chronic toxicity study, methanolic bark extracts of E. agallocha did not produce any significant changes in body weight of mice model when compared to both control groups (negative and positive) of mice. Moreover, there was significant rising of serum SGPT level during the treatment with the plant extract at a drug 400 mg/kg, but no significant changes were observed with 200 mg/kg dose, indicating no acute or sub-chronic toxicity of the extract. In conclusion, E. agallocha bark extract can be considered as a potential anticancer compound which should be validated from other animal model trials. Further studies are required to fractionate the extract, to identify the bioactive compounds, and which may be directed to carry out in vivo studies of its medicinal active components. Sharmin Sultana M. Biotechnology 2020-08-06T10:18:52Z 2020-08-06T10:18:52Z 2019 2019-10 Thesis ID 18176002 http://hdl.handle.net/10361/13907 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 130 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Kupchan method Antioxidant activity Anticancer activity Hemotological parameters Thin layer chromatography |
| spellingShingle |
Kupchan method Antioxidant activity Anticancer activity Hemotological parameters Thin layer chromatography Sultana, Sharmin Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract |
| description |
This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2019. |
| author2 |
Hossain, Mahboob |
| author_facet |
Hossain, Mahboob Sultana, Sharmin |
| format |
Thesis |
| author |
Sultana, Sharmin |
| author_sort |
Sultana, Sharmin |
| title |
Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract |
| title_short |
Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract |
| title_full |
Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract |
| title_fullStr |
Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract |
| title_full_unstemmed |
Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract |
| title_sort |
evaluation of the antioxidant and in vivo anticancer activity of excoecaria agallocha bark extract |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13907 |
| work_keys_str_mv |
AT sultanasharmin evaluationoftheantioxidantandinvivoanticanceractivityofexcoecariaagallochabarkextract |
| _version_ |
1814307794950553600 |