Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019.
| Tác giả chính: | |
|---|---|
| Tác giả khác: | |
| Định dạng: | Luận văn |
| Ngôn ngữ: | English |
| Được phát hành: |
Brac University
2020
|
| Những chủ đề: | |
| Truy cập trực tuyến: | http://hdl.handle.net/10361/13906 |
| id |
10361-13906 |
|---|---|
| record_format |
dspace |
| spelling |
10361-139062020-08-06T21:01:10Z Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city Mostafa, Fariha Binte Siddiqee, Mahbubul H Department of Mathematics and Natural Sciences, Brac University Horizontal Gene Transfer (HGT) Surface water Dhaka city Trans-gram transformations Transgenic organisms. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. Cataloged from PDF version of thesis. Includes bibliographical references (pages 46-49). A global medical crisis has already started as antibiotics have started losing their effectiveness against the growing number of bacterial pathogens. Horizontal Gene Transfer (HGT) system has a great impact on this spreading of antibiotic resistance. However, the transformation dynamic is poorly understood. To find out the condition of surface water of Dhaka city that, how favorable it is for transformation of antibiotic resistance gene, Gulshan Lake and Hatirjheel Lake were chosen for this experiment, where natural transformation was performed between trans-gram bacteria Staphylococcus aureus and Salmonella typhi. From the 10 isolates, that were collected from BRAC university laboratory, 3 highest resistance showing isolates of Salmonella (Salmonella GL6.2, Salmonella HJ & Salmonella HJ3.1) were chosen as donor and one ATCC strain of Staphylococcus aureus was confirmed as recipient by the Kirby-Buer disk diffusion method. Finally, transformation was performed in Gulshan lake water and in hatirjheel lake water mixing the chromosomal DNA (Boiling method) and plasmid DNA (Kado-Liu method) of donor with recipient. Further, the confirmation test of transformation showed that, no DNA was uptake in any sample of the Gulshan and Hatirjheel lake water against any of the two specific antibiotics (Azithromycin & Tetracycline). The findings from this experiment indicates that, though horizontal gene transfer has a lot of impact in the spread of antibiotic resistance, spreading through natural transformation between gram positive and gram-negative organism might not be a very common phenomenon. At this time of rapid spreading of antibiotic resistance, this finding will help us to think in different ways of possible trans-gram transformations. Fariha Binte Mostafa B. Biotechnology 2020-08-06T09:54:51Z 2020-08-06T09:54:51Z 2019 2019-10 Thesis ID 15336011 http://hdl.handle.net/10361/13906 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 55 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Horizontal Gene Transfer (HGT) Surface water Dhaka city Trans-gram transformations Transgenic organisms. |
| spellingShingle |
Horizontal Gene Transfer (HGT) Surface water Dhaka city Trans-gram transformations Transgenic organisms. Mostafa, Fariha Binte Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. |
| author2 |
Siddiqee, Mahbubul H |
| author_facet |
Siddiqee, Mahbubul H Mostafa, Fariha Binte |
| format |
Thesis |
| author |
Mostafa, Fariha Binte |
| author_sort |
Mostafa, Fariha Binte |
| title |
Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city |
| title_short |
Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city |
| title_full |
Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city |
| title_fullStr |
Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city |
| title_full_unstemmed |
Horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of Dhaka city |
| title_sort |
horizontal transfer of antibiotic resistance between trans-gram bacteria in surface water of dhaka city |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13906 |
| work_keys_str_mv |
AT mostafafarihabinte horizontaltransferofantibioticresistancebetweentransgrambacteriainsurfacewaterofdhakacity |
| _version_ |
1814306989114654720 |