Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019.
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Brac University
2020
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| Accés en línia: | http://hdl.handle.net/10361/13905 |
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10361-139052020-08-06T21:01:18Z Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital Marha, Faria Hossain, Mahboob Mahmud, Zahid Hayat Department of Mathematics and Natural Sciences, Brac University HA-MRSA MDR-MRSA Hand sanitizers MecA femA nuc HAI PCR Methicillin resistance Staphylococcus aureus infections This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. Cataloged from PDF version of thesis. Includes bibliographical references (pages 31-36). Methicillin-Resistant Staphylococcus aureus (MRSA) is notorious for its resistance to Penicillin derivative antibiotics and is also resistant to multiple drugs. In Bangladesh, the prevalence of MRSA is a staggering 72%. This study takes an approach to investigate the incidence, molecular characterization and antibiotic profiling of MRSA from clinical as well as associated surroundings in hospitals. Initial screening was done using selective and differential media and then confirmed by PCR against nuc, mecA and femA gene. The nuc+ isolates were tested against various antibiotic classes to produce an antibiotic profile. Isolates taken from hand swabs were tested for their susceptibility against hand sanitizers. 68.9% of the isolates in this study were proved to be MRSA through detection of nuc, femA and mecA and subsequent disk diffusion test revealed that 90.9% of those isolates were non-susceptible to multiple drugs and surprisingly a 100% of the isolate found on hand were unresponsive to hand sanitizers. Currently, we are at the cusp of a time when to combat the emergence of such a Hospital Associated Infection (HAI) causative superbug extensive research into its cultural along with molecular machinery is imperative. This thesis aims to investigate the incidence of MRSA in hospitalized patients and their immediate surroundings and their characterization. Faria Marha B. Biotechnology 2020-08-06T09:42:46Z 2020-08-06T09:42:46Z 2019 2019-10 Thesis ID 15136021 http://hdl.handle.net/10361/13905 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 36 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
HA-MRSA MDR-MRSA Hand sanitizers MecA femA nuc HAI PCR Methicillin resistance Staphylococcus aureus infections |
| spellingShingle |
HA-MRSA MDR-MRSA Hand sanitizers MecA femA nuc HAI PCR Methicillin resistance Staphylococcus aureus infections Marha, Faria Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. |
| author2 |
Hossain, Mahboob |
| author_facet |
Hossain, Mahboob Marha, Faria |
| format |
Thesis |
| author |
Marha, Faria |
| author_sort |
Marha, Faria |
| title |
Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital |
| title_short |
Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital |
| title_full |
Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital |
| title_fullStr |
Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital |
| title_full_unstemmed |
Incidence, molecular characterization and antibiotic resistance profile of Methicillin resistant staphylococcus aureus Isolated from clinical origin and associated surroundings in hospital |
| title_sort |
incidence, molecular characterization and antibiotic resistance profile of methicillin resistant staphylococcus aureus isolated from clinical origin and associated surroundings in hospital |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13905 |
| work_keys_str_mv |
AT marhafaria incidencemolecularcharacterizationandantibioticresistanceprofileofmethicillinresistantstaphylococcusaureusisolatedfromclinicaloriginandassociatedsurroundingsinhospital |
| _version_ |
1814307556094377984 |