Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019.
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| বিন্যাস: | গবেষণাপত্র |
| ভাষা: | English |
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Brac University
2020
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| অনলাইন ব্যবহার করুন: | http://hdl.handle.net/10361/13904 |
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10361-139042020-08-06T21:01:21Z Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris Abreshmi, Afra Hossain, Mahboob Mannoor, Kaiissar Department of Mathematics and Natural Sciences, Brac University P. pastoris HBsAg Vaccine AOX promoter F1R1 primers F2R2 primers Vaccination--Complications Hepatitis B--Vaccination This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. Cataloged from PDF version of thesis. Includes bibliographical references (pages 32-34). Hepatitis B is a potentially life-threatening liver infection caused by the Hepatitis B virus. It is a major health problem worldwide. So, the development of safe and effective vaccines against this disease can be a huge triumph for mankind. Pichia. pastoris is a suitable host for inducing the production of the Hepatitis B surface antigen (HBsAg) inside it’s body as well as it can be used for commercially large scale production. This can be done by inserting the HBsAg gene into the plasmid vector of P. pastoris yeast under the alcohol oxidase (AOX) promoter and let them grow in suitable condition. Our study was conducted to ensure the insertion of the HBsAg gene under the AOX promoter into the yeast P. pastoris after allowing them to be stored as Master Cell Bank (MCB) and Working Cell Bank (WCB). Thus, the first aim of this study was to characterize the Master Cell Bank and Working Cell Bank by Phenotypic tests; like microbial purity, identity and viability. Secondly, HBsAg gene in P. pastoris was analyzed by polymerase chain reaction (PCR) using two sets of primers named F1R1 and F2R2, followed by performing gel electrophoresis and Sanger sequencing. Characterization of the cell banks as well as ensuring the recombinant HBsAg gene indicates that visually all the P. pastoris contain the HBsAg gene. Finally, the main objective of this study was to ensure the expression, purification, and characterization of HBsAg in yeast P. pastoris. The study was carried out successfully. Afra Abreshmi B. Biotechnology 2020-08-06T09:23:29Z 2020-08-06T09:23:29Z 2019 2019-10 Thesis ID 15136009 http://hdl.handle.net/10361/13904 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 34 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
P. pastoris HBsAg Vaccine AOX promoter F1R1 primers F2R2 primers Vaccination--Complications Hepatitis B--Vaccination |
| spellingShingle |
P. pastoris HBsAg Vaccine AOX promoter F1R1 primers F2R2 primers Vaccination--Complications Hepatitis B--Vaccination Abreshmi, Afra Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. |
| author2 |
Hossain, Mahboob |
| author_facet |
Hossain, Mahboob Abreshmi, Afra |
| format |
Thesis |
| author |
Abreshmi, Afra |
| author_sort |
Abreshmi, Afra |
| title |
Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris |
| title_short |
Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris |
| title_full |
Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris |
| title_fullStr |
Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris |
| title_full_unstemmed |
Characterization of Hepatitis B surface Antigen gene containing recombinant Pichia pastoris |
| title_sort |
characterization of hepatitis b surface antigen gene containing recombinant pichia pastoris |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13904 |
| work_keys_str_mv |
AT abreshmiafra characterizationofhepatitisbsurfaceantigengenecontainingrecombinantpichiapastoris |
| _version_ |
1814307794780684288 |