HPLC method development for the analysis of linagliptin
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy, 2019.
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| 語言: | English |
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Brac University
2020
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| 在線閱讀: | http://hdl.handle.net/10361/13772 |
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10361-13772 |
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10361-137722020-02-17T21:01:15Z HPLC method development for the analysis of linagliptin Mimiya, Ruhani Amrin Kabir, Eva Rahman Department of Pharmacy, Brac University Diabetes mellitus DPP-4 inhibitors Linagliptin High performance liquid chromatography This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy, 2019. Cataloged from PDF version of thesis report. Includes bibliographical references (pages 54-60). The purpose of this study was to develop a reliable, rapid and sensitive method based on high performance liquid chromatography (HPLC) for the determination of an anti-diabetic drug linagliptin in its pure form as well as in its pharmaceutical dosage forms. Different chromatographic conditions were investigated to optimize the parameters for the established method. In this study, the chromatographic assay involved the use of C18 column (150×4.6mm, packed with 5 μm particles) as the stationary phase and a combination of methanol: phosphate buffer (of pH 4.5) as the mobile phase at a ratio of 70:30 v/v pumped at a flow rate of 1mL/min in isocratic mode. The total run time was 10 min with a retention time of 3.3 min. The detection wavelength was 241 nm. Five marketed products of linagliptin were assayed by this developed method and their potency were calculated successfully, the results of which demonstrated both the feasibility and reliability of this method for the quantitation and qualification of linagliptin in pharmaceutical dosage forms as well as in its pure form. Ruhani Amrin Mimiya B. Pharmacy 2020-02-17T10:10:26Z 2020-02-17T10:10:26Z 2019 2019-05 Thesis ID 14346010 http://hdl.handle.net/10361/13772 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Diabetes mellitus DPP-4 inhibitors Linagliptin High performance liquid chromatography |
| spellingShingle |
Diabetes mellitus DPP-4 inhibitors Linagliptin High performance liquid chromatography Mimiya, Ruhani Amrin HPLC method development for the analysis of linagliptin |
| description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy, 2019. |
| author2 |
Kabir, Eva Rahman |
| author_facet |
Kabir, Eva Rahman Mimiya, Ruhani Amrin |
| format |
Thesis |
| author |
Mimiya, Ruhani Amrin |
| author_sort |
Mimiya, Ruhani Amrin |
| title |
HPLC method development for the analysis of linagliptin |
| title_short |
HPLC method development for the analysis of linagliptin |
| title_full |
HPLC method development for the analysis of linagliptin |
| title_fullStr |
HPLC method development for the analysis of linagliptin |
| title_full_unstemmed |
HPLC method development for the analysis of linagliptin |
| title_sort |
hplc method development for the analysis of linagliptin |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13772 |
| work_keys_str_mv |
AT mimiyaruhaniamrin hplcmethoddevelopmentfortheanalysisoflinagliptin |
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