Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019.
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Brac University
2020
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10361-137622020-02-16T21:01:22Z Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme Tamanna, Shams Tahsin Hossain, Mahboob Department of Mathematics and Natural Sciences, Brac University Alpha (α)-amylase Enzyme Soil microorganisms This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019. Catalogued from PDF version of thesis. Includes bibliographical references (pages 74-81). Alpha (α)-amylase is an enzyme responsible for the breakdown of starch molecules. It hydrolyzes starch into reducing and fermentable sugars like maltose. The enzyme has various industrial applications; namely in food, detergent, leather, paper, chemical, and pharmaceutical industries. In this study, α-amylase producing bacteria were isolated from two soil samples taken from two different places of Dhanmondi, Dhaka. Out of 23 isolates from the soil samples, 10 could hydrolyze starch and were selected for identification and characterization. The selected isolates were gram-positive Bacillus and Solibacillus; and gram-negative Raoultella and Klebsiella. Antibiotic susceptibility of the 10 isolates was determined by the Kirby-Bauer test. Gram-negative isolates were more resistant to antibiotics than the gram-positive isolates. Among the selected isolates, Bacillus cibi showed the highest antimicrobial activity against pathogenic strains. The effects of temperature, pH and salinity on the growth of isolates were determined. The optimum growth temperature for the isolates ranged from 370C to 400C, and optimal pH for growth ranged from 7.0 to 7.5. The isolates were subjected to submerged fermentation for the production of α-amylase and the activities of extracted amylase enzymes were determined by the DNS test. Maximum amylase activity were displayed by Bacillus subtilis (0.49 unit/ml), Bacillus amyloliquefaciens (0.46 unit/ml), and Bacillus sporothermodurans (0.44 unit/ml). The isolates were further characterized by studying the effects of temperature, pH, and presence of various chemical agents and metallic salts on the activity of bacterial amylase. The enzymes showed maximum activity at temperature 370C~400C, and pH 6.0~6.7. Enzyme activity was increased in the presence of calcium. However, it was decreased in the presence of chemical agents like urea, EDTA and acetic acid. Shams Tahsin Tamanna B. Microbiology 2020-02-16T09:30:37Z 2020-02-16T09:30:37Z 2019 2019-11 Thesis ID 15126002 http://hdl.handle.net/10361/13762 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 84 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Alpha (α)-amylase Enzyme Soil microorganisms |
| spellingShingle |
Alpha (α)-amylase Enzyme Soil microorganisms Tamanna, Shams Tahsin Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme |
| description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019. |
| author2 |
Hossain, Mahboob |
| author_facet |
Hossain, Mahboob Tamanna, Shams Tahsin |
| format |
Thesis |
| author |
Tamanna, Shams Tahsin |
| author_sort |
Tamanna, Shams Tahsin |
| title |
Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme |
| title_short |
Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme |
| title_full |
Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme |
| title_fullStr |
Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme |
| title_full_unstemmed |
Isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-Amylase enzyme |
| title_sort |
isolation and identification of α-amylase producing soil microorganisms and partial characterization of α-amylase enzyme |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13762 |
| work_keys_str_mv |
AT tamannashamstahsin isolationandidentificationofaamylaseproducingsoilmicroorganismsandpartialcharacterizationofaamylaseenzyme |
| _version_ |
1814306921065218048 |