Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019.
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | |
| التنسيق: | أطروحة |
| اللغة: | English |
| منشور في: |
Brac University
2020
|
| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/13761 |
| id |
10361-13761 |
|---|---|
| record_format |
dspace |
| spelling |
10361-137612020-02-16T21:01:21Z Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing Fatima, Sehnin Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Antibiotics Serratia marcescens Prodigiosin Antibacterial activity Microbial pigments Secondary metabolites This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019. Catalogued from PDF version of thesis. Includes bibliographical references (pages 42-45). Antibiotic resistance has become a challenging issue in medical treatment process. A large portion the novel antibiotics are derived from soil microbes as soil being a primary source. Hence, to isolate novel antibiotic agent, soil sample was screened in several steps and one isolate showed antimicrobial activity against the growth of two out of ten selected the test organisms (Bacillus licheniformis and Bacillus subtilis). The isolated bacteria was later identified as Serratia sp. Genotypic characterization was later carried out by amplifying the 16S rRNA gene of the isolate using universal primers in PCR mehtod. The amplified 16S rRNA gene of the isolate was sequenced using Sanger sequencing method and compared to the NCBI nucleotide database. The isolate had highest similarities with Serratia marcescens subsp. sakuensis. The isolate showed good antimicrobial activity but requires further characterization to determine its potentiality to be used as a new antibiotic for commercial production. Sehnin Fatima B. Microbiology 2020-02-16T09:02:53Z 2020-02-16T09:02:53Z 2019 2019-09 Thesis ID 15126022 http://hdl.handle.net/10361/13761 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 46 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Antibiotics Serratia marcescens Prodigiosin Antibacterial activity Microbial pigments Secondary metabolites |
| spellingShingle |
Antibiotics Serratia marcescens Prodigiosin Antibacterial activity Microbial pigments Secondary metabolites Fatima, Sehnin Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing |
| description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019. |
| author2 |
Haque, Fahim Kabir Monjurul |
| author_facet |
Haque, Fahim Kabir Monjurul Fatima, Sehnin |
| format |
Thesis |
| author |
Fatima, Sehnin |
| author_sort |
Fatima, Sehnin |
| title |
Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing |
| title_short |
Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing |
| title_full |
Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing |
| title_fullStr |
Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing |
| title_full_unstemmed |
Isolation of antibiotic producing bacteria from soil and identification by 16S rRNA gene sequencing |
| title_sort |
isolation of antibiotic producing bacteria from soil and identification by 16s rrna gene sequencing |
| publisher |
Brac University |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13761 |
| work_keys_str_mv |
AT fatimasehnin isolationofantibioticproducingbacteriafromsoilandidentificationby16srrnagenesequencing |
| _version_ |
1814308464199991296 |