Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018.
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| Định dạng: | Luận văn |
| Ngôn ngữ: | English |
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BRAC
2020
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| Truy cập trực tuyến: | http://hdl.handle.net/10361/13520 |
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10361-135202020-01-15T21:01:16Z Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital Amin, Md. Ruhul Hossain, M. Mahboob Department of Mathematics and Natural Sciences, Brac University Dhaka Medical College Hospital This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (pages 43-55). New Delhi Metallo-beta-lactamase-1 (NDM-1) producing superbugs create a global public health problem because of their resistance to most of the antibiotics. This study was conducted to determine the presence of NDM-1 producers in carbapenem-resistant Pseudomonas aeruginosa isolated from Dhaka Medical College Hospital, Bangladesh. Out of 120 gram-negative bacteria isolated from wound swab, 58 (48.33%) were Pseudomonas aeruginosa, 28 of which were from burn unit and 30 were from medicine, surgery, gynecology and obstetrics units. Pseudomonas aeruginosa isolated from burn unit showed more resistance to all other antibiotics than organism isolated from other units. Extended spectrum-β-lactamase (ESBL) producers were detected by double disk synergy test (DDS) and Metallo-β-lactamase (MBL) producers were detected by both Hodge test and double disk synergy test. Total 38 (31.67%) isolates were detected as ESBL producers and 40 (33.33%) were detected as MBL producers. In the case of Pseudomonas aeruginosa 17 (29.31%) were ESBL producers and 20 (34.48%) were MBL producers. Total 24 (41.37%) carbapenem-resistant Pseudomonas aeruginosa were detected by the disk diffusion test of which 15 were from burn unit and 9 from other units. Carbapenem-resistant Pseudomonas aeruginosa were screened for the presence of NDM-1 by PCR and 6 (25%) were found positive for blaNDM-1. In the case of the burn unit, 33.33% of carbapenem-resistant Pseudomonas aeruginosa were NDM-1 producer. NDM-1 producing Pseudomonas aeruginosa were found 100% resistant to all commonly used antibiotics except colistin. The result of this study provided insights into the high proportion of NDM-1 producers among Pseudomonas aeruginosa in Dhaka Medical College Hospital and the importance of rational use of antibiotics. Md. Ruhul Amin B. Biotechnology 2020-01-15T06:14:43Z 2020-01-15T06:14:43Z 2018 2018-12 Thesis ID 14276003 http://hdl.handle.net/10361/13520 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 61 pages application/pdf BRAC |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Dhaka Medical College Hospital |
| spellingShingle |
Dhaka Medical College Hospital Amin, Md. Ruhul Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. |
| author2 |
Hossain, M. Mahboob |
| author_facet |
Hossain, M. Mahboob Amin, Md. Ruhul |
| format |
Thesis |
| author |
Amin, Md. Ruhul |
| author_sort |
Amin, Md. Ruhul |
| title |
Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital |
| title_short |
Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital |
| title_full |
Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital |
| title_fullStr |
Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital |
| title_full_unstemmed |
Detection of new Delhi Metallo-β-lactamase-1 (NDM-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from Dhaka Medical College Hospital |
| title_sort |
detection of new delhi metallo-β-lactamase-1 (ndm-1) gene from multidrug-resistant pseudomonas aeruginosa isolated from dhaka medical college hospital |
| publisher |
BRAC |
| publishDate |
2020 |
| url |
http://hdl.handle.net/10361/13520 |
| work_keys_str_mv |
AT aminmdruhul detectionofnewdelhimetalloblactamase1ndm1genefrommultidrugresistantpseudomonasaeruginosaisolatedfromdhakamedicalcollegehospital |
| _version_ |
1814307923329810432 |