Horizontal transfer of antibiotic resistance in surface water of Dhaka City
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019.
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| বিন্যাস: | গবেষণাপত্র |
| ভাষা: | English |
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Brac University
2019
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| অনলাইন ব্যবহার করুন: | http://hdl.handle.net/10361/12308 |
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10361-123082019-09-30T05:53:09Z Horizontal transfer of antibiotic resistance in surface water of Dhaka City Paul, Jitu Siddiqee, Mahbubul Hasan Siddique, Romana Department of Mathematics and Natural Sciences, Brac University Horizontal gene transfer Antibiotic resistance Kirby-Bauer Disk Diffusion method Plasmid curing Microbial growth Culture media (Biology) This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. Cataloged from PDF version of thesis. Includes bibliographical references (pages 45-47). Horizontal gene transfer is one of the most widespread phenomenon by which bacteria evolve themselves in the environment. Such transfer is often reported to be responsible for spreading antibiotic resistance genes in nature. This study was designed to understand the significance of natural transformation among different bacterial species in the surface water of Dhaka city in the light of emergence of antibiotic resistance; Salmonella sp. and Klebsiella sp. were used as model donor and recipient respectively. A total 10 environmental Salmonella isolates and 5 potential recipients (ATCC strains) were collected from BRAC University Microbiology Lab. By using Kirby-Bauer Disk Diffusion method, donor and recipient strains were selected. Among the 10 Salmonella isolates, 3 (Salmonella GL6.2, Salmonella HJ & Salmonella HJ3.1) showed highest number of resistant against 9 different antibiotics and these isolates were considered as a highly resistant donor for this experiment. Whereas, among those 5 ATCC stains, Klebsiella variicola showed highest level of sensitivity against 9 different antibiotics and this strain was selected for this study. After the antibiotic susceptibility test was done, the chromosomal DNA (boiling method) and plasmid DNA (Kado-Liu method) of the donor Salmonella strains were extracted for bacterial natural transformation experiment. Obtained results indicate that, the rate of chromosomal DNA mediated transformation is higher than plasmid DNA mediated transformation between Salmonella spp. and Klebsiella variicola. Further, it has been found that among the two matrices tested in this study (waters from Hatirjheel and Gulshan lakes), bacterial transformation is higher in Gulshan Lake; 34 successful transformed-bacterial cell growth found on antibiotic containing LB plates out of 90 different cases as opposed to 23 successful transformed-bacterial cell growth in Hatirjheel surface water. Further assays indicate that transfer of antibiotic resistance mostly involves chromosomal DNA-mediated transformation. Overall, this study reveals that horizontal gene transfer in surface waters could be one of the significant factors behind the rise of antibiotic resistance in Dhaka. Jitu Paul B. Biotechnology 2019-07-04T06:46:55Z 2019-07-04T06:46:55Z 2019 2019-05 Thesis ID 14236009 http://hdl.handle.net/10361/12308 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 52 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Horizontal gene transfer Antibiotic resistance Kirby-Bauer Disk Diffusion method Plasmid curing Microbial growth Culture media (Biology) |
| spellingShingle |
Horizontal gene transfer Antibiotic resistance Kirby-Bauer Disk Diffusion method Plasmid curing Microbial growth Culture media (Biology) Paul, Jitu Horizontal transfer of antibiotic resistance in surface water of Dhaka City |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. |
| author2 |
Siddiqee, Mahbubul Hasan |
| author_facet |
Siddiqee, Mahbubul Hasan Paul, Jitu |
| format |
Thesis |
| author |
Paul, Jitu |
| author_sort |
Paul, Jitu |
| title |
Horizontal transfer of antibiotic resistance in surface water of Dhaka City |
| title_short |
Horizontal transfer of antibiotic resistance in surface water of Dhaka City |
| title_full |
Horizontal transfer of antibiotic resistance in surface water of Dhaka City |
| title_fullStr |
Horizontal transfer of antibiotic resistance in surface water of Dhaka City |
| title_full_unstemmed |
Horizontal transfer of antibiotic resistance in surface water of Dhaka City |
| title_sort |
horizontal transfer of antibiotic resistance in surface water of dhaka city |
| publisher |
Brac University |
| publishDate |
2019 |
| url |
http://hdl.handle.net/10361/12308 |
| work_keys_str_mv |
AT pauljitu horizontaltransferofantibioticresistanceinsurfacewaterofdhakacity |
| _version_ |
1814308608658112512 |