Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh
This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2015.
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BRAC University
2019
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| Online Erişim: | http://hdl.handle.net/10361/12258 |
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10361-122582019-09-30T06:00:14Z Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh Rahat, Asifuzzaman Qadri, Firdausi Choudhury, Naiyyum Department of Mathematics and Natural Sciences, BRAC University Antibiotic sensivity Acute lower respiratory tract infection (ARI) Multi-hospital surveillance Respiratory therapy. Respiratory infections--Prevention. This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2015. Catalogued from PDF version of thesis report. Includes bibliographical references (pages 101-111). Acute lower respiratory tract infection (ARI), enteric fever and diarrhea remain the prevalent causes for mortality and morbidity of under-five children in Bangladesh. However, little information is available on true prevalence of the pathogens responsible for these diseases in under-five of Bangladesh and their susceptibility to antibiotics. In this work, we evaluated the bacterial causes of infection in children seeking care in two hospitals in Dhaka. This study utilized specimens collected as part of routine diagnostic purpose encompassing, nasal swab for ARI, stool for diarrhea, and blood for enteric fever. For isolation of the bacterial pathogens, conventional microbiological culture methods were used, followed by detection using appropriate biochemical and serological tests. For enteric fever, an additional immunological detection of Salmonella Typhi and Paratyphi specific IgA immune response in lymphocyte secretions by Typhoid-Paratyphoid Test (TPTest) was done and results compared with that of blood culture. For diarrheal samples, ETEC isolates were tested for specific toxins using multiplex PCR. Antibiotic susceptibility tests were performed according to the Clinical and Laboratory Standard Institute (CLSI) guidelines. Of 100 ARI samples analyzed, 30 bacteria of different species were isolated, of which S. pneumoniae (50%) and K. pneumoniae (26.7%) were the most prevalent. The overall antibiotic sensitivity test of the isolates showed Meropenem, Impenem, Ceftriaxone, Ciprofloxacin, Penicillin and Ampicillin as the most potent antibiotic against respiratory isolates. Of 47 samples tested for enteric fever, 1 (2.1%) was culture positive for Salmonella Typhi, while 23 (49%) were TPTest positive. The S. Typhi isolate was only resistant to Nalidixic acid. 5 of 17 (29.4%) diarrheal samples were culture positive, 3 of which were identified as ETEC and the other two V. cholerae, and Shigella dysenteriae respectively. The findings from this study elucidate the major aetiologies causing infection in under-five children in Dhaka. The antimicrobial sensitivity pattern of the relevant pathogens unraveled will help guide physicians to prescribe appropriate antibiotics in settings where diagnostic facilities are limited or not available. Asifuzzaman Rahat M. Biotechnology 2019-06-25T10:45:58Z 2019-06-25T10:45:58Z 2015 2015-07-27 Thesis ID 13276002 http://hdl.handle.net/10361/12258 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 111 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Antibiotic sensivity Acute lower respiratory tract infection (ARI) Multi-hospital surveillance Respiratory therapy. Respiratory infections--Prevention. |
| spellingShingle |
Antibiotic sensivity Acute lower respiratory tract infection (ARI) Multi-hospital surveillance Respiratory therapy. Respiratory infections--Prevention. Rahat, Asifuzzaman Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh |
| description |
This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2015. |
| author2 |
Qadri, Firdausi |
| author_facet |
Qadri, Firdausi Rahat, Asifuzzaman |
| format |
Thesis |
| author |
Rahat, Asifuzzaman |
| author_sort |
Rahat, Asifuzzaman |
| title |
Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh |
| title_short |
Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh |
| title_full |
Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh |
| title_fullStr |
Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh |
| title_full_unstemmed |
Multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in Dhaka, Bangladesh |
| title_sort |
multi-hospital surveillance and antibiotic sensitivity among bacterial isolates of acute respiratory and enteric infection in underfive children in dhaka, bangladesh |
| publisher |
BRAC University |
| publishDate |
2019 |
| url |
http://hdl.handle.net/10361/12258 |
| work_keys_str_mv |
AT rahatasifuzzaman multihospitalsurveillanceandantibioticsensitivityamongbacterialisolatesofacuterespiratoryandentericinfectioninunderfivechildrenindhakabangladesh |
| _version_ |
1814307340684361728 |