Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019.
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Brac University
2019
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| Sarrera elektronikoa: | http://hdl.handle.net/10361/12081 |
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10361-12081 |
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10361-120812019-09-30T05:33:47Z Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils George, Romi Marina Hossain, Dr. Mahboob Department of Mathematics and Natural Sciences, Brac University Biodegradation Soil microbiology. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. Cataloged from PDF version of thesis. Includes bibliographical references (page 52-57). Microorganisms play a very important role in the biological decomposition of various materials in the natural environment, this is called biodegradation. Synthetic materials including plastic and polyethylene waste accumulate in the environment and pose an ever-increasing ecological threat to humankind and the overall ecosystem on earth. Biodegradation of these plastic wastes using potent microbial strains could provide a solution to the problem. In the present study, the microorganisms responsible for biodegradation were isolated and characterized from local samples contaminated with hydrocarbon polymers. The experiment was conducted over a series of screening methods. The primary screening involved culturing collected samples for over 40 days to screen out low-density polyethylene (LDPE) non-degrading bacteria, followed by observation of polyethylene glycol (PEG) utilization by the formation of a clear zone. This led to the isolation of 43 different colonies. Secondary screening further allowed for selection of 14 different isolates that were able to form biofilm over the polymer surface. Finally, the tertiary screening allowed for the selection of 10 different isolates that are confirmed LDPE-degraders. From continuous culturing for 36 days, 8 out of 10 isolates were able to survive and propagate in minimal salt broth, namely, Staphylococcus sp., Acinetobacter sp., Clostridium sp., Bacillus sp., and Lysinibacillus sp. Finally, organism able to produce anionic and cationic biosurfactant were identified as Clostridium novyitype A and Staphylococcus massiliensis respectively. Romi Marina George B. Biotechnology 2019-05-21T05:34:35Z 2019-05-21T05:34:35Z 2019 2019-03 Thesis ID 14236008 http://hdl.handle.net/10361/12081 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 57 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biodegradation Soil microbiology. |
| spellingShingle |
Biodegradation Soil microbiology. George, Romi Marina Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. |
| author2 |
Hossain, Dr. Mahboob |
| author_facet |
Hossain, Dr. Mahboob George, Romi Marina |
| format |
Thesis |
| author |
George, Romi Marina |
| author_sort |
George, Romi Marina |
| title |
Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| title_short |
Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| title_full |
Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| title_fullStr |
Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| title_full_unstemmed |
Isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| title_sort |
isolation and characterization of low-density polyethylene degrading and biosurfactant-producing bacteria from soils |
| publisher |
Brac University |
| publishDate |
2019 |
| url |
http://hdl.handle.net/10361/12081 |
| work_keys_str_mv |
AT georgeromimarina isolationandcharacterizationoflowdensitypolyethylenedegradingandbiosurfactantproducingbacteriafromsoils |
| _version_ |
1814307257930743808 |