Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018.
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2019
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10361-110642019-09-30T04:42:23Z Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk Saha, Bishakha Joyeeta Hossain, Dr. M. Mahboob Department of Mathematics and Natural Sciences, BRAC University Multidrug resistant PCR Animal modeling SDS-PAGE Pathogenesis Enteric infection This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (page 46-48). Pseudomonas is a kind of bacteria that has the ability to develop resistance to antibiotics rather rapidly over several generations. This resistance present in some strains makes Pseudomonas a very difficult to treat once a host, such as a human or other animal, is infected. The purpose of this study was to analyze the antibiotic resistant genes of Pseudomonas spp. through molecular techniques along with the assessment of enteric pathogenesis using animal modeling. Primarily, the isolates were characterized morphologically and identified by 16S rRNA as Pseudomonas spp. Presence of antibiotic resistant genes were tested for 8 screened isolates from antibiogram from 25 environmental (Soil), Clinical and UHT milk isolates using specific oligonucleotide primers (VIM 2-F & VIM 2-R, IMP 1-F & IMP 1-R, OXA 2-F & OXA 2-R) in gradient PCR. Enteric pathogenetic nature of the isolates was tested using two standard animal models. Out of 25 isolates, 8 isolates showed high multidrug resistance and the presence of antibiotic resistant genes were confirmed by amplifying 865bp, 587bp and 810bp DNA fragments respectively in PCR. Total cellular partially purified protein extracted from 8 pathogenic multidrug resistant isolates selected from PCR, tested for SDS PAGE to produce patterns with wide range of discrete bands with specific molecular weight. Presences of antibiotic resistant genes were confirmed by PCR and no protein was detected by SDS-PAGE according to specific molecular weight. Enteric pathogenesis was observed from the rabbit ileal loop fluid accumulation and physiological changes followed by direct consumption in rats. Detection of pathogenic multidrug resistant microbial strains can lead us to a solution for outbreak of enteric infection. Bishakha Joyeeta Saha B. Biotechnology 2019-01-02T05:19:01Z 2019-01-02T05:19:01Z 2018 2018-09 Thesis ID 13236016 http://hdl.handle.net/10361/11064 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 55 pages application/pdf |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Multidrug resistant PCR Animal modeling SDS-PAGE Pathogenesis Enteric infection |
| spellingShingle |
Multidrug resistant PCR Animal modeling SDS-PAGE Pathogenesis Enteric infection Saha, Bishakha Joyeeta Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk |
| description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. |
| author2 |
Hossain, Dr. M. Mahboob |
| author_facet |
Hossain, Dr. M. Mahboob Saha, Bishakha Joyeeta |
| format |
Thesis |
| author |
Saha, Bishakha Joyeeta |
| author_sort |
Saha, Bishakha Joyeeta |
| title |
Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk |
| title_short |
Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk |
| title_full |
Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk |
| title_fullStr |
Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk |
| title_full_unstemmed |
Analysis of enteric pathogenicity of multidrug-resistant Pseudomonas spp. isolated from commercial UHT milk |
| title_sort |
analysis of enteric pathogenicity of multidrug-resistant pseudomonas spp. isolated from commercial uht milk |
| publishDate |
2019 |
| url |
http://hdl.handle.net/10361/11064 |
| work_keys_str_mv |
AT sahabishakhajoyeeta analysisofentericpathogenicityofmultidrugresistantpseudomonassppisolatedfromcommercialuhtmilk |
| _version_ |
1814307338521149440 |