Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018.
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BRAC University
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10361-109142019-09-30T06:12:16Z Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety Parvez, Aakib Siddiqee, Mahbubul Haque Department of Mathematics and Natural Sciences, BRAC University Antimicrobial property Garlic Herbal plants This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018. Catalogued from PDF version of thesis report. Includes bibliographical references (page 46). Natural antibiotics or commonly known as herbal plants are used against various small diseases throughout the history of mankind. Now we use synthetic antibiotics. However, these antibiotics are very much ineffective against various microorganisms because of the antibiotic resistance. In current work we used Bangladeshi and hybrid garlic against Vibrio cholerae, Staphylococcus aureus, Bacillus cereus and Streptococcus pyogenes. The garlic samples were dried and ethanolic and methanolic extract were prepared following a previously published protocol. The extracts were diluted by Dimethyl sulfoxide and Distilled water at 75%, 50% and 25% for conducting agar diffusion tests. The prepared extracts were tested on microorganisms by using minimal bactericidal concentration of garlic extract. Further, agar well diffusion test were performed in order to determine the zone of inhibition. For determine the qualitative analysis of the phytochemical properties of Bangladeshi and hybrid garlic, we did basic phytochemical screening tests. Bangladeshi variety of garlic showed MBC of 62.5%, 67.5%, 52.5%, and 65% against Vibrio cholerae, Staphylococcus aureus, Bacillus cereus and Streptococcus pyogenes respectively where as MBC of 70%, 75%, 70%, and 70% was found for the hybrid variety against the same organisms. The average diameters of inhibition-zone were 11mm, 12.5mm, 21.5mm and 18.65mm against Vibrio cholerae, Staphylococcus aureus, Bacillus cereus and Streptococcus pyogenes respectively while the same for hybrid variety were 8mm, 6mm, 20.67mm, and 7mm These results suggest that the local variety might be more effective against the bacteria tested in this study. The phytochemical test result shows that Bangladeshi Garlic contains protiens, tannins and phenols, alkaloids and tritenpenoids on the other hand hybrid garlic has proteins and alkaloids but tritenpenoids was absent. Overall, the result shows that Bangladeshi garlic might be more effective against microorganisms and it can act as a stepping stone to use garlic for therapeutic purposes. Aakib Paevez B. Microbiology 2018-11-29T09:24:43Z 2018-11-29T09:24:43Z 2018 2018-07 Thesis ID 13326009 http://hdl.handle.net/10361/10914 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 50 pages application/pdf BRAC University |
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Brac University |
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Institutional Repository |
language |
English |
topic |
Antimicrobial property Garlic Herbal plants |
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Antimicrobial property Garlic Herbal plants Parvez, Aakib Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety |
description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018. |
author2 |
Siddiqee, Mahbubul Haque |
author_facet |
Siddiqee, Mahbubul Haque Parvez, Aakib |
format |
Thesis |
author |
Parvez, Aakib |
author_sort |
Parvez, Aakib |
title |
Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety |
title_short |
Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety |
title_full |
Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety |
title_fullStr |
Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety |
title_full_unstemmed |
Local varieties of Allium Sativum has more effective antimicrobial property compared to imported variety |
title_sort |
local varieties of allium sativum has more effective antimicrobial property compared to imported variety |
publisher |
BRAC University |
publishDate |
2018 |
url |
http://hdl.handle.net/10361/10914 |
work_keys_str_mv |
AT parvezaakib localvarietiesofalliumsativumhasmoreeffectiveantimicrobialpropertycomparedtoimportedvariety |
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1814309416096235520 |