Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018.
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BRAC University
2018
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10361-107962019-09-30T05:57:34Z Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism Islam, Syeda Sumaiya Siddiqee, Mahbubul Hasan Department of Mathematics and Natural Sciences, BRAC University Human pathogens Vibrio cholerae Surface water Vibrio infections Cholera Vibrio cholerae This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018. Catalogued from PDF version of thesis report. Includes bibliographical references (page 38-41) Urbanization and industrialization results in the fecal organisms contaminating the water-bodies which are important sources of recreational activities. This eventually increases the risk of humans getting infected during accidental ingestion or contact. The purpose of the study was to detect the presence of Vibrio cholerae in water bodies contaminated with industrial pollutants. For this purpose Hatirjheel Lake and Buriganga River, the two prominent water bodies of our country were chosen for sampling. A total of 40 samples were collected aseptically from Hatirjheel Lake and 20 samples from Buriganga River within duration of three months. Then, they were processed for isolation of culturable Vibrio cholerae. After that several biochemical tests were done for presumptive identification of isolates. For further confirmation, RT-PCR was done targeting CqsS gene. While many isolates were found to be positive with biochemical tests, after RT-PCR only 5% of samples from both water bodies gave positive result. Further, three strains from both water bodies were tested for survival potential under starvation stress. The survival patterns between the lake and river showed slight difference but both showed a gradual decrease in culturable count over the time. Next, the strains from those water bodies were checked for their susceptibility to an array of eight antibiotics. The antibiogram revealed that isolates from Hatirjheel showed complete resistance to tetracycline, ampicillin, ceftriaxone while the Buriganga isolates showed complete resistance to only ampicillin, tetracycline, nalidixic acid, co-trimethoprim and ceftriaxone. While no conclusion could be drawn on the actual presence of Vibrio cholerae in Hatirjheel or Buriganga, isolation of this organism from heavily polluted water bodies could be difficult. Syeda Sumaiya Islam B. Microbiology 2018-11-04T04:47:04Z 2018-11-04T04:47:04Z 2018 2018 Thesis ID 13226008 http://hdl.handle.net/10361/10796 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 50 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Human pathogens Vibrio cholerae Surface water Vibrio infections Cholera Vibrio cholerae |
| spellingShingle |
Human pathogens Vibrio cholerae Surface water Vibrio infections Cholera Vibrio cholerae Islam, Syeda Sumaiya Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism |
| description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018. |
| author2 |
Siddiqee, Mahbubul Hasan |
| author_facet |
Siddiqee, Mahbubul Hasan Islam, Syeda Sumaiya |
| format |
Thesis |
| author |
Islam, Syeda Sumaiya |
| author_sort |
Islam, Syeda Sumaiya |
| title |
Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism |
| title_short |
Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism |
| title_full |
Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism |
| title_fullStr |
Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism |
| title_full_unstemmed |
Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism |
| title_sort |
understanding the effect of exposure of human pathogens to polluted surface water: vibrio cholerae as a model organism |
| publisher |
BRAC University |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/10796 |
| work_keys_str_mv |
AT islamsyedasumaiya understandingtheeffectofexposureofhumanpathogenstopollutedsurfacewatervibriocholeraeasamodelorganism |
| _version_ |
1814309345991589888 |